文献类型：期刊文献

中文题名：养殖密度对墨吉明对虾肠道和生物絮团菌群的影响

英文题名：Effects of stocking density on bacterial community characterization of biofloc and intestine of Fenneropenaeus merguiensis

作者：王博[1];秦海鹏[1];廖栩峥[1];胡世康[1];赵吉臣[1];何子豪[1];韩学明[1];陈兆明[1];孙成波[1]

机构：[1]广东海洋大学水产学院,广东湛江524088

年份：2020

卷号：46

期号：5

起止页码：608

中文期刊名：湖南农业大学学报（自然科学版）

外文期刊名：Journal of Hunan Agricultural University(Natural Sciences)

收录：CSTPCD、、北大核心2017、CSCD2019_2020、北大核心、CSCD

基金：2019年度“冲一流”广东省财政专项资金项目(231419025);防城港市科技计划项目(防科AD19008017)。

语种：中文

中文关键词：墨吉明对虾;密度胁迫;生物絮团;肠道;菌群结构

外文关键词：Fenneropenaeus merguiensis;stocking density;biofloc;intestine;bacterial community

中文摘要：运用Miseq高通量测序技术,分析高、中、低3种养殖密度(700、300、100尾/m3)下墨吉明对虾肠道和其养殖系统中生物絮团的菌群结构。结果显示:对虾肠道菌群中,高密度组的丰富度和多样性均最高,丰富度随放养密度的增大而增加,而在生物絮团菌群中,高密度组的丰富度最低;在门水平上,肠道和絮团样本菌群均以变形菌门(相对丰度49.25%~80.33%)、放线菌门、拟杆菌门、绿弯菌门为主,但丰度差异较大;在属水平上,优势菌属的组成和所占比例明显不同,梭菌属、Spongiibacter、Faecalibacterium、Rhodovulum、Blautia仅在肠道样本中存在,Coccinimonas仅在絮团样本中存在;肠道样本中,中密度组弧菌属的相对丰度最小,为5.23%,高密度组弧菌属的相对丰度最大,为23.39%,絮团样本中,低密度组弧菌属的相对丰度最大,为16.15%,中、高密度弧菌属的相对丰度均较小,分别为5.51%和4.20%;随养殖密度增大,肠道样本中拟杆菌属的相对丰度减小,分别为1.36%、0.41%、0.02%,而不同密度絮团样本拟杆菌属的相对丰度差异不明显。可见,生物絮团养殖模式下,养殖密度对墨吉明对虾的肠道和絮团菌群的影响较大。

外文摘要：Using the high-throughput sequencing technology of Miseq,the microbial structure of the intestine and water biofloc of Fenneropenaeus merguiensis at high,medium and low aquaculture densities(700/m3,300/m3,100/m3)were analyzed.The results showed that among the shrimp intestinal flora,the high-density group had the highest abundance and diversity,and the abundance increased over the increase of stocking density,while among the biofloc flora,the high-density group had the lowest abundance.The intestinal and biofloc flora samples were mainly composed of Proteobacteria(relative abundant 49.25%~80.33%),Actinobacteria,Bacteroides and Chloroflexi at phylum level,but the abundance was different.At genus level,the composition and proportion of dominant bacteria were significantly different.Clostridium,Spongiibacter,Faecalibacterium,Rhodovulum,and Blautia only exist in intestinal samples,and Coccinimonas only exist in biofloc samples.The relative abundance of Vibrio was relatively large,Vibrio abundance of intestinal sample was the smallest(5.23%)in medium density group and the largest(23.39%)in high density group;the low-density group of biofloc samples was the largest(16.15%),and the medium and high-density samples were both smaller,the abundance was 5.51%and 4.20%,respectively.The abundance of Bacteroides in the intestinal samples decreased with the increase of stocking density,and the abundance was 1.36%,0.41%,0.02%,respectively.The abundance difference of Bacteroides in different culture density biofloc samples was not significant.It can be seen that under the bioflocculation mode,the density of culture had a greater influence on bacterial community characterization of biofloc and intestine of Fenneropenaeus merguiensi.