文献类型：期刊文献

中文题名：饲料中添加甘露寡糖对珍珠龙胆石斑鱼生长性能、血清免疫指标、转录组及肠道菌群的影响

英文题名：Effects of Dietary Mannan-Oligosaccharides on Growth Performance,Serum Immune Indices,Transcriptome and Intestinal Microflora of Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂

作者：王红明[1];丁雪婧[1];陈俭[1];宋守钢[1];谭北平[1,2];章双[1,2]

机构：[1]广东海洋大学水产动物营养与饲料实验室,湛江524088;[2]农业部华南水产与畜禽饲料重点实验室,湛江524088

年份：2021

卷号：33

期号：12

起止页码：6982

中文期刊名：动物营养学报

外文期刊名：CHINESE JOURNAL OF ANIMAL NUTRITION

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：国家重点研发计划项目(2019YFD0900200)。

语种：中文

中文关键词：甘露寡糖;珍珠龙胆石斑鱼;生长性能;免疫;转录组;肠道菌群

外文关键词：mannan-oligosaccharides;Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂;growth performance;immune;transcriptome;intestinal microflora

中文摘要：本试验旨在研究饲料中添加甘露寡糖对珍珠龙胆石斑鱼(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂)生长性能、血清免疫指标、转录组及肠道菌群的影响。选择240尾初始平均体重为(6.00±0.32)g的珍珠龙胆石斑鱼幼鱼,随机分为2组,每组3个重复,每个重复40尾鱼。对照组饲喂基础饲料,甘露寡糖组在基础饲料中添加100 mg/kg甘露寡糖。养殖8周后,分析甘露寡糖对珍珠龙胆石斑鱼生长性能、血清免疫指标、转录组和肠道菌群的影响。结果显示:1)甘露寡糖组的终末体重、增重率、特定生长率和肥满度均显著高于对照组(P<0.05)。2)甘露寡糖组的血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、溶菌酶(LZM)和碱性磷酸酶(AKP)活性显著高于对照组(P<0.05),血清丙二醛(MDA)含量显著低于对照组(P<0.05)。3)转录组测序分析共鉴定出9964个差异表达基因,其中3801个表达上调,6163个表达下调。GO功能富集分析结果表明,差异表达基因在生物过程、细胞组成和分子功能显著富集。KEEG通路富集分析发现,差异表达基因在吞噬体、细胞黏附分子、细胞因子-细胞因子受体相互作用、免疫球蛋白A产生的肠道免疫网络相关通路中显著富集。4)甘露寡糖组肠道菌群的操作分类单元(OTU)数量、Ace指数、Chaol指数和Shannon指数均显著高于对照组(P<0.05)。甘露寡糖组肠道拟杆菌门和厚壁菌门相对丰度显著高于对照组(P<0.05)。甘露寡糖组肠道发光杆菌属相对丰度显著低于对照组(P<0.05),而肠道德沃斯氏菌属、盐单胞菌属和甲基杆菌属相对丰度显著高于对照组(P<0.05)。综上所述,饲料中添加100 mg/kg甘露寡糖可促进珍珠龙胆石斑鱼的生长,提高免疫功能,改善肠道菌群结构。

外文摘要：This experiment was conducted to study the effects of dietary mannan-oligosaccharides on growth performance,serum immune indices,transcriptome and intestinal microflora of Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂.A total of 240 juvenile Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂with initial average body weight of(6.00±0.32) g were randomly divided into 2 groups with 3 replicates per group and 40 fish per replicate.Fish in the control group were fed a basal diet,and others in mannan-oligosaccharides group were fed the basal diet supplemented with 100 mg/kg mannan-oligosaccharides.After 8 weeks breeding,the effects of dietary mannan-oligosaccharides on growth performance,serum immune indices,transcriptome and intestinal microflora of Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂ were analyzed.The results showed as follows:1) the final body weight,weight gain rate,specific growth rate and condition factor of mannan-oligosaccharides group were significantly higher than those of the control group(P<0.05).2) The activities of superoxide dismutase(SOD),catalase(CAT),lysozyme(LZM) and alkaline phosphatase(AKP) in serum of mannan-oligosaccharides group were significantly higher than those of the control group(P<0.05),while the serum malondialdehyde(MDA) content was significantly lower than that of the control group(P<0.05).3) Transcriptome sequencing analysis identified 9 964 differentially expressed genes,which 3 801 were up-regulated and 6 163 were down-regulated.GO functional enrichment analysis revealed that the differentially expressed genes were significantly enriched in biological process,molecular function and cell component.KEEG pathway enrichment analysis differentially expressed genes in phagosome,cell adhesion molecules,cytokine-cytokine receptor interactions and immunoglobulin A-producing intestinal immune network related pathways were significantly enriched.4) The operational taxonomic unit(OTU) number,Ace index,Chaol index and Shannon index of intestinal microflora of mannan-oligosaccharides group were significantly higher than those of the control group(P<0.05).The relative abundances of Bacteroidetes and Firmicutes in intestine of mannan-oligosaccharides group were significantly higher than those of the control group(P<0.05).The relative abundances of Photobacterium in intestine of mannan-oligosaccharides group was significantly lower than that of the control group(P<0.05),and the relative abundances of Devosia,Halomonas and Methylobacterium in intestine were significantly higher than those of the control group(P<0.05).In conclusion,dietary 100 mg/kg mannan-oligosaccharides can significantly promote the growth performance of Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂,improve the immune function,and improve the intestinal microflora structure.