文献类型：期刊文献

英文题名：The Inhibition Effect of the Seaweed Polyphenol, 7-Phloro-Eckol from Ecklonia Cava on Alcohol-Induced Oxidative Stress in HepG2/CYP2E1 Cells

作者：Lin, Liyuan[1,2];Yang, Shengtao[1,2];Xiao, Zhenbang[1,2];Hong, Pengzhi[1,2];Sun, Shengli[1];Zhou, Chunxia[1,2];Qian, Zhong-Ji[1,2]

机构：[1]Guangdong Ocean Univ, Shenzhen Inst, Coll Food Sci & Technol, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab, Zhanjiang 524088, Peoples R China

年份：2021

卷号：19

期号：3

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000633858300001)、、Scopus(收录号：2-s2.0-85103862402)、WOS

基金：The research was funded by the 2020 Shenzhen International Scientific and Technological Cooperation R&D Project and the Natural Science Foundation of Guangdong Province (2020A1515011075). The supported by the Development Project about Marine Economy Demonstration of Zhanjiang City (XM-202008-01B1) and Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang, ZJW-2019-07).

语种：英文

外文关键词：7-phloro-eckol; HepG2; oxidative stress; apoptosis

外文摘要：The liver is vulnerable to oxidative stress-induced damage, which leads to many diseases, including alcoholic liver disease (ALD). Liver disease endanger people's health, and the incidence of ALD is increasing; therefore, prevention is very important. 7-phloro-eckol (7PE) is a seaweed polyphenol, which was isolated from Ecklonia cava in a previous study. In this study, the antioxidative stress effect of 7PE on HepG2/CYP2E1 cells was evaluated by alcohol-induced cytotoxicity, DNA damage, and expression of related inflammation and apoptosis proteins. The results showed that 7PE caused alcohol-induced cytotoxicity to abate, reduced the amount of reactive oxygen species (ROS) and nitric oxide (NO), and effectively inhibited DNA damage in HepG2/CYP2E1 cells. Additionally, the expression levels of glutathione (GSH), superoxide dismutase (SOD), B cell lymphoma 2 (Bcl-2), and Akt increased, while gamma-glutamyltransferase (GGT), Bcl-2 related x (Bax), cleaved caspase-3, cleaved caspase-9, nuclear factor-kappa B (NF-kappa B), and JNK decreased. Finally, molecular docking proved that 7PE could bind to BCL-2 and GSH protein. These results indicate that 7PE can alleviate the alcohol-induced oxidative stress injury of HepG2 cells and that 7PE may have a potential application prospect in the future development of antioxidants.