文献类型：期刊文献

中文题名：响应面法优化BacillusamyloliquefaciensZJHD-06产类细菌素发酵培养基

英文题名：Optimization of fermentation medium of Bacillus amyloliquefaciens ZJHD-06 by response surface methodology

作者：安俊莹[1];刘颖[1];朱雯娟[1];胡雪琼[1];叶莉珍[1]

机构：[1]广东省水产品加工与安全重点实验室,广东省普通高等学校水产品深加工重点实验室,广东海洋大学食品科技学院,广东湛江524000

年份：2014

卷号：35

期号：1

起止页码：191

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD、、北大核心2011、北大核心、CSCD、CSCD2013_2014

基金：广东省自然科学基金(S2011010000328);广东省科学技术厅(粤科规划字[2009]198号)

语种：中文

中文关键词：响应面;类细菌素;发酵培养基

外文关键词：response surface methology; bacteriocin-like substance;fermentation medium

中文摘要：以分离自海洋宝石石斑鱼肠道的一种解淀粉芽孢杆菌ZJHD-06所产类细菌素为研究对象,以单核增生李斯特菌为抗菌活性测试指示菌,以抑菌圈直径大小为指标,采用响应面法对菌株ZJHD-06产类细菌素的发酵培养基成分进行优化。通过Plackett-Burman实验筛选出对类细菌素产量具有显著影响的因素,再通过最陡爬坡实验确定显著因素的中心水平,最后进行Box-Behnken实验设计,最终得到的最佳配比为葡萄糖浓度4.80%,蛋白胨浓度0.47%,酵母浸膏浓度0.44%,NaCl、K2HPO4和MgSO4·7H2O浓度分别为1.0%、0.01%、0.03%。在该培养基配比下,发酵上清液的抑菌圈直径增加了27.8%。

外文摘要：The fermentation medium of Bacillus amyloliquefacien ZJHD-06 isolated from intestine of Epinephelus areolatus was optimized for a bacteriocin-fike substance（BLS）production.Bacteriostatic circle diameter was used as evaluation index against L.monocytogenes ATCC 35152.Firstly,the notable factors were screened by Plackett- Burman experiment design.Then,the path of steepest ascent was undertaken to approach the optimal region of the significant factors.Finally,Box-Behnken design was adopted to identify optimal proportion.The results showed the optimal proportions were glucose 4.80%, peptone 0.47%, yeast extract 0.44%, NaCl 1.0%, K2HPO＂ 0.01% and MgSQ-TH20 0.03%.The bacteriostatic circle diameter of the fermentation supernatant was increased by 2？.8% using the optimal proportions.