文献类型：期刊文献

中文题名：基于纳米银颗粒团聚反应的表面增强拉曼光谱法测定牛奶中三聚氰胺的含量

英文题名：Determination of melamine in milk based on silver nanoparticles aggregation by surface enhanced raman spectroscopy

作者：冯彦婷[1];林沛纯[1];谢慧风[2,3];王玲[1];李承勇[2,3]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]广东海洋大学深圳研究院,广东深圳518018;[3]广东海洋大学化学与环境学院,广东湛江524088

年份：2019

卷号：45

期号：15

起止页码：256

中文期刊名：食品与发酵工业

外文期刊名：Food and Fermentation Industries

收录：CSTPCD、、北大核心2017、CSCD2019_2020、北大核心、CSCD

基金：国家自然科学基金(21874029);深圳市大鹏新区产业发展专项资金(KY20170209,KY20180202)

语种：中文

中文关键词：表面增强拉曼;表面等离子体共振;三聚氰胺;快速检测

外文关键词：surface enhanced raman;surface plasmon resonance;melamine;rapid detection

中文摘要：建立表面增强拉曼(surface-enhanced raman scattering,SERS)技术检测牛奶中三聚氰胺含量的方法。以金属钛板作为SERS衬底材料,采用50 nm银纳米颗粒作为基底,控制银溶胶与样品的体积比为1∶2,NaCl和NaOH溶液的浓度为4 mol/L,通过便携式拉曼光谱仪采集样品的SERS信号。在质量浓度0.2~10 mg/L的范围内,SERS强度随着牛奶中三聚氰胺浓度的增大而增强,线性相关系数 R2=0.998,检测限为0.08 mg/L。使用银纳米基底对1 mg/L加标样品平行测定20次,三聚氰胺特征峰强度的相对标准偏差为4.34%。此法简单易行,重现性好、稳定性高,可实现对牛奶中三聚氰胺含量的快速检测,为食品中污染物的SERS检测提供了参考价值。

外文摘要：The surface-enhanced raman scattering (SERS) technique was used to detect melamine in milk. The metal titanium plate was used as a SERS substrate material, 50 nm silver nanoparticles were substrates, the ratio of the silver nanoparticles to the sample was1 ∶2, and concentrations of both NaCl and NaOH were 4 mol/L. The SERS signal was acquired by a portable raman spectrometer. It was found that the intensity of SERS increased as the concentration of melamine increased from 0.2 mg/L to10 mg/L ( R2=0.998). Moreover, the detection limit was 0.08 mg/L and the relative standard deviation of the characteristic peak intensity of melamine was 4.34%. The established method was simple and easy to perform with good reproducibility and high stability. It can realize rapid detection of melamine in milk, which provides a reference for applying SERS in detecting pollutants in foods.