文献类型：期刊文献

英文题名：Exogenous Spermidine Enhances Drought Resistance of Mango Seedlings by Regulating Physiological and Biochemical Metabolism

作者：Liu, Xinyu[1,2];Wang, Mingtian[3,4];Yan, Jing[2];Cheng, Feng[2];Liao, Wei[2];Xiao, Yunhe[2];Zhou, Lirong[1];Zhang, Meng[1];Leng, Xiangchi[1];Liang, Qingzhi[1]

机构：[1]Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang 524088, Peoples R China;[2]Renhe Meteorol Bur Panzhihua, Key Lab Disaster Weather Def & Climate Resource, Panzhihua 617000, Peoples R China;[3]Water Saving Agr Res Southern Hilly Area Key Lab S, Chengdu 610066, Peoples R China;[4]Sichuan Meteorol Observ, Chengdu 610072, Peoples R China

年份：2025

卷号：11

期号：9

外文期刊名：HORTICULTURAE

收录：SCI-EXPANDED(收录号：WOS:001580029200001)、、WOS

基金：This work was funded by the Natural Science Foundation of Hainan Province (324MS102), Panzhihua City Guiding Science and Technology Plan Project (2024ZD-N-38), Natural Science Foundation of China (31471849) and Program for Scientific Research Start-up Funds of Gongdong Ocean University (030301052410).

语种：英文

外文关键词：drought stress; spermidine; Mangifera indica L.

外文摘要：Drought stress is a major environmental factor that adversely affects plant growth and development. Spermidine (SPD), a polyamine, plays a critical role in plant defense mechanisms against drought stress. PEG was used to simulate osmotic stress, which mimics drought conditions under controlled environments. This study investigated the effects of exogenous spermidine (SPD) on the physiological and biochemical responses of mango plants under drought stress and explored its potential mitigation mechanisms. Two-year-old 'Renong 1' mango seedlings were subjected to drought stress induced by polyethylene glycol (PEG 6000) at concentrations of 5%, 15%, and 25%, simulating mild, moderate, and severe drought conditions, respectively. Plants were subsequently treated with 1 mmol/L spermidine. After PEG 6000 treatment and spermidine application for 3 days, the leaf morphology, relative chlorophyll content, malondialdehyde (MDA) levels, antioxidant enzyme activities (superoxide dismutase [SOD], peroxidase [POD], catalase [CAT]), and osmotic regulators (proline, soluble sugars, and soluble proteins) were analyzed. The results demonstrated that drought stress caused leaf chlorosis, desiccation, reduced relative chlorophyll content, elevated MDA levels (indicating lipid peroxidation), enhanced antioxidant enzyme activities, increased proline and soluble sugar accumulation for osmotic regulation, and decreased soluble protein content. Exogenous spermidine treatment significantly alleviated drought-induced damage by reducing leaf chlorosis, delaying relative chlorophyll degradation (by 20.0-25.7% under moderate drought and 14.1-19.1% under severe drought), and decreasing MDA levels (by 4.8-9.5% under moderate drought and 0.8-23.7% under severe drought). Furthermore, spermidine enhanced antioxidant enzyme activities (e.g., SOD activity increased by 24.9-37.4% and POD by 74.0-104.0% under moderate drought), regulated osmotic substance accumulation (e.g., proline decreased by 21%, 26%, and 24% under mild, moderate, and severe drought, respectively), and mitigated the reduction in soluble protein content (by 6.6% under moderate drought and 10.3% under severe drought). In conclusion, exogenous spermidine mitigates drought-induced damage in mango by preserving photosynthetic capacity, enhancing the antioxidant defense system, and modulating osmotic balance. These results showed that SPD could significantly improve plant vigor or survival rate under stress. It provides a theoretical basis for water-saving cultivation of mango, improving the stress resistance of mango varieties and the application of spermidine in tropical fruit production.