文献类型：期刊文献

英文题名：Nicotinamide mononucleotide improves oocyte quality by reprogramming ovarian metabolism, increasing estrogen synthesis, reducing oxidative stress, and inhibiting apoptosis in the spotted scat (Scatophagus argus)

作者：Luo, Jie[1,2]; Wang, Siqin[1,2]; Lin, Xinghua[1,2]; Wang, Tuo[3]; Li, Jinli[1]; Jiang, Dongneng[1,2]; Wu, Tianli[1,2]; Deng, Siping[1,2,4]

机构：[1] Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China; [2] Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Zhanjiang, 524088, China; [3] College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, 524088, China; [4] Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, 524088, China

年份：2026

外文期刊名：SSRN

收录：EI(收录号：20260062450)

语种：英文

外文关键词：Blood - Cell membranes - Cholesterol - Gene expression - Insulin - Mammals - Metabolism - Mitochondria - Oxidative stress

外文摘要：Oocyte quality is essential for reproductive success in teleost fish. Nicotinamide mononucleotide (NMN), a precursor of nicotinamide adenine dinucleotide (NAD+) can improve oocyte quality in mammals. To investigate the effects of NMN on oocyte quality in the spotted scat (Scatophagus argus), levels of serum glucose, insulin, total cholesterol, triglycerides, 17β-estradiol (E2), and ovarian NAD+ were analyzed following an intraperitoneal (IP) injection of NMN. Ovarian transcriptomic profiling was also conducted using the RNA-sequencing. The expression of genes related to steroidogenesis, apoptosis, and oxidative stress after NMN supplementation was analyzed by qRT-PCR. Additionally, the ATP content, glutathione (GSH) levels, reactive oxygen species (ROS) accumulation, and mitochondrial membrane potential (ΔΨm) were quantified in the follicles after NMN incubation. The results revealed that NMN injection significantly increased ovarian NAD+ bioavailability and serum E2 levels. NMN improved glucose and lipid metabolism, as evidenced by elevated insulin levels and reduced serum glucose, total cholesterol, and triglycerides. KEGG enrichment analysis revealed significant enrichment of pathways related to metabolism, stress response, and apoptosis. qRT-PCR demonstrated the upregulation of steroidogenesis-related genes (cyp17a1, hsd3b1, 20β-hsd, cyp19a1a, mmp2, foxl2), antioxidant genes (gpx1a, gpx1b, slc7a11), and the anti-apoptotic gene bcl2, as well as the the downregulation of pro-apoptotic genes (bax, caspase3). NMN incubation attenuated oxidative stress by decreasing ROS accumulation and increasing GSH levels, while enhancing mitochondrial function through elevated ΔΨm and ATP production. In conclusion, NMN supplementation improves oocyte quality by reprogramming ovarian metabolism, promoting steroidogenesis, mitigating oxidative stress, and inhibiting apoptosis in spotted scat. ? 2026, The Authors. All rights reserved.