文献类型：期刊文献

英文题名：A simple and sensitive HPLC method based on pre-column fluorescence labelling for multiple classes of plant growth regulator determination in food samples

作者：Li, Guoliang[1,3];Liu, Shucheng[3];Sun, Zhiwei[1];Xia, Lian[1];Chen, Guang[1];You, Jinmao[1,2]

机构：[1]Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China;[2]Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China;[3]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang, Peoples R China

年份：2015

卷号：170

起止页码：123

外文期刊名：FOOD CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000343780400017)、、EI(收录号：20143718157667)、Scopus(收录号：2-s2.0-84906968570)、WOS

基金：This work was supported by The National Natural Science Foundation of China (Nos. 31301595, 21275089 and 21305076), Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety (GDPKLAPPS1401), The Scientific Research Fund of Qufu Normal University (xkj201302) and Ph.D. research start-up funds of Qufu Normal University (BSQD2012017, BSQD20110119).

语种：英文

外文关键词：Plant growth regulators; Pre-column fluorescence labelling; HPLC; Food samples

外文摘要：The determination of trace plant growth regulator (PGR) has received more and more attentions in the field of phytophysiology and food safety. But the simple and sensitive method for simultaneously analysing multiple classes of PGR remains poorly investigated. In this study, a new pre-column fluorescence labelling method using 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methylbenzenesulfonate (BCETS) as the labelling reagent has been developed for simultaneous determination of seven PGRs (i.e., indole-3-acetic acid, 3-indolybutyric acid, 3-indolepropionic acid, jasmonic acid, gibberellin A(3), 1-naphthylacetic acid and 2-naphthaleneacetic acid) by HPLC with fluorescent detection (FLD). The proposed method offered the LOD of 0.34-0.73 ng/mL for seven PGRs, which were significantly lower than the reported methods. The crude extract without complex pre-treatments and purification was directly labelled by BCETS and analysed by HPLC-FLD, which facilitates the high-throughput sample screening. This method was proven to be inexpensive, simple, selective, sensitive, accurate and reliable for trace PGR determination. (C) 2014 Elsevier Ltd. All rights reserved.