文献类型：期刊文献

英文题名：Lipidomic insights into the immune response and pearl formation in transplanted pearl oyster Pinctada fucata martensii

作者：Wu, Hailing[1];Yang, Chuangye[1];Hao, Ruijuan[2];Liao, Yongshan[3,4];Wang, Qingheng[1,3,4,5];Deng, Yuewen[1,3,4,5]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhanjiang, Dev & Res Ctr Biol Marine Resources, Zhanjiang, Peoples R China;[3]Guangdong Ocean Univ, Guangdong Sci & Innovat Ctr Pearl Culture, Zhanjiang, Peoples R China;[4]Guangdong Ocean Univ, Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang, Peoples R China;[5]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000874680200001)、、WOS

基金：This work was supported by National Natural Science Foundation of China (Grant No. 32102817), Guangdong Basic and Applied Basic Research Foundation (Grant No. 2022A1515010030), Department of Education of Guangdong Province (Grant No. 2019KQNCX043, 2020ZDZX1045 and 2021KCXTD026), and the earmarked fund for CARS-49.

语种：英文

外文关键词：Pinctada fucata martensii; lipidomics; transplantation; immune response; pearl formation

外文摘要：During pearl culture, the excess immune responses may induce nucleus rejection and death of pearl oysters after transplantation. To better understand the immune response and pearl formation, lipidomic analysis was applied to investigate changes in the serum lipid profile of pearl oyster Pinctada fucata martensii following transplantation. In total, 296 lipid species were identified by absolute quantitation. During wound healing, the content of TG and DG initially increased and then decreased after 3 days of transplantation with no significant differences, while the level of C22:6 decreased significantly on days 1 and 3. In the early stages of transplantation, sphingosine was upregulated, whereas PC and PUFAs were downregulated in transplanted pearl oyster. PI was upregulated during pearl sac development stages. GP and LC-PUFA levels were upregulated during pearl formation stage. In order to identify enriched metabolic pathways, pathway enrichment analysis was conducted. Five metabolic pathways were found significantly enriched, namely glycosylphosphatidylinositol-anchor biosynthesis, glycerophospholipid metabolism, alpha-linolenic acid metabolism, linoleic acid metabolism and arachidonic acid metabolism. Herein, results suggested that the lipids involved in immune response, pearl sac maturation, and pearl formation in the host pearl oyster after transplantation, which might lead to an improvement in the survival rate and pearl quality of transplanted pearl oyster.