文献类型：期刊文献

英文题名：Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (Sillago sihama)

作者：Saetan, Wanida[1,2];Tian, Changxu[1,2];Yu, Jiawang[1];Lin, Xinghua[1,2];He, Feixiang[1,2];Huang, Yang[1,2,3];Shi, Hongjuan[1,2,3];Zhang, Yulei[1,2,3];Li, Guangli[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Guangdong Res Ctr Reprod Control & Breeding Techn, Zhanjiang 524088, Peoples R China;[3]Marine Ecol & Aquaculture Environm Zhanjiang, Zhanjiang 524088, Peoples R China

年份：2020

卷号：10

期号：4

外文期刊名：ANIMALS

收录：SCI-EXPANDED(收录号：WOS:000531832800085)、、WOS

基金：This study was supported by grants from the National Natural Science Foundation of China (Nos. 41706174 and 31702326), Natural Science Foundation of Guangdong Province (2016A030313743, 2017A030313101, 2018B030311050 and Edited based on previous published papers in the journal. 2019A1515010958), Independent Project of Guangdong Province Laboratory (ZJW-2019-06), the Department of Education of Guangdong Province (2018KQNCX111 and 2019KTSCX060) and Program for Scientific Research Start-up Funds of Guangdong Ocean University (R19026).

语种：英文

外文关键词：Sillago sihama; hypoxia stress response; gene expression; RNA-Seq

外文摘要：Simple Summary Silver sillago (Sillago sihama) is a marine fish species with a high economic value. S. sihama is poorly resistant to hypoxia. However, hypoxia stress-related genes and pathways in S. sihama remain unclear. In this study, we compared gill tissues of S. sihama between hypoxia and normoxia groups and detected differentially expressed genes under hypoxia stress. Two gene families, such as cytochrome P450 and glutathione S-transferase were associated with the function of metabolic process under the hypoxia stress. This study will expand our knowledge about the molecular mechanism of the transcriptome response to hypoxia stress in S. sihama. Abstract Silver sillago (Sillago sihama) is a commercially important marine fish species in East Asia. In this study, we compared the transcriptome response to hypoxia stress in the gill tissue of S. sihama. The fish were divided into four groups, such as 1 h of hypoxia (hypoxia1h, DO = 1.5 +/- 0.1 mg/L), 4 h of hypoxia (hypoxia4h, DO = 1.5 +/- 0.1 mg/L), 4 h of reoxygen (reoxygen4h, DO = 8.0 +/- 0.2 mg/L) after 4 h of hypoxia (DO = 1.5 mg/L), and normoxia or control (DO = 8.0 +/- 0.2 mg/L) groups. Compared to the normoxia group, a total of 3550 genes were identified as differentially expressed genes (DEGs) (log(2)foldchange > 1 and padj < 0.05), including 1103, 1451 and 996 genes in hypoxia1h, hypoxia4h and reoxygen4h groups, respectively. Only 247 DEGs were differentially co-expressed in all treatment groups. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, DEGs were significantly enriched in steroid biosynthesis, biosynthesis of amino acids, glutathione metabolism and metabolism of xenobiotics by cytochrome P450, ferroptosis and drug metabolism-cytochrome P450 pathways. Of these, the cytochrome P450 (CYP) and glutathione S-transferase (GST) gene families were widely expressed. Our study represents the insights into the underlying molecular mechanisms of hypoxia stress.