文献类型：期刊文献

英文题名：Molecular characterization of OSR1 in Pinctada fucata martensii and association of allelic variants with growth traits

作者：Yang, Chuangye[1,2];Yang, Jingmiao[1];Hao, Ruijuan[1];Du, Xiaodong[1,2];Deng, Yuewen[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Pearl Breeding & Proc Engn Technol Res Ctr Guangd, Zhanjiang 524088, Peoples R China

年份：2020

卷号：516

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000501480700044)、、WOS

基金：This work was supported by "Innovation Team Project" (Grant number: 2017KCXTD016) from the Department of Education of Guangdong Province, the Science and Technology Department of Guangdong Province (Grant number: 2018A030310666, 2017A030307024 and 2017A030303076), the China Agriculture Research System (Grant number: CARS-049).

语种：英文

外文关键词：Growth traits; Odd-skipped related 1; Pinctada fucata martensii; Single nucleotide polymorphisms

外文摘要：Odd-skipped related 1 (OSR1) from Pinctada fucata martensii (PmOSR1) was cloned in this study. PmOSR1 contained an open reading frame measuring 951 bp that encoded a polypeptide of 316 residues. Domain analysis also revealed that PmOSR1 had a typical ZnF_C2H2 domain, indicating high identity with OSR1 protein sequence from Crassostrea gigas (55.25%) and Mizuhopecten yessoensis (65.48%). The expression pattern analysis of PmOSR1 revealed its wide expression in the tissues and developmental stages. PmOSR1 also presented a higher expression in the fast-growing group and hybrid families than in the slow-growing group and inbred families. The polymorphism analysis of PmOSR1 revealed 45 single nucleotide polymorphisms (SNPs) in the genomic sequence, and association analysis presented four SNPs significantly associated with growth traits (namely, shell length, width, height, and shell weight; P < 0.05). Three SNPs out of the four SNPs were shaped as a haploblock, and haplotype AGT showed significantly higher growth traits than that of haplotype AACAA. Therefore, PmOSR1 could be used as a growth candidate gene for the selective breeding of P. f. martensii.