文献类型：期刊文献

英文题名：Toxicity of Chronic Waterborne Zinc Exposure in Pacific White Shrimp Litopenaeus Vannamei

作者：Liang, Zhi[1,2]; Chen, Tianci[1,2]; Yang, Furong[1,2]; Li, ShuHong[1,2]; Guo, Hui[1,2]

机构：[1] College of Fisheries, Guangdong Ocean University, Zhanjiang, 524025, China; [2] Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524025, China

年份：2022

外文期刊名：SSRN

收录：EI(收录号：20220276060)

语种：英文

外文关键词：Cell death - Detoxification - Gene expression - Growth rate - Industrial poisons - Polymerase chain reaction - Risk assessment - Zinc

外文摘要：Zinc (Zn) is an essential metal for aquatic animals, but its excessive accumulation has potentially toxic effects. The present study was performed to explore the physiological changes and molecular mechanism involved in Zn exposure at its environmentally relevant concentrations on the hepatopancreas of white shrimp ?Litopenaeus vannamei , based on the? biochemical ,? morphological, ?and transcriptional alterations. In this study, we investigated the toxic effect of Zn on the hepatopancreas of the? L. vannamei ?by exposing shrimp to 0 (control), 0.01, 0.1, and 1 mg/L Zn for 30 days. The results showed that the weight gain (WG) and specific growth rate (SGR) of? L. vannamei ?were significantly decreased with the increase of ambient Zn, whereas the 0.01 mg/L Zn exposure promoted hepatopancreas growth. Zn decreased the activities of trypsin, and amylase (AMS), as well as total antioxidant capacity (T-AOC). Moreover, exposure to Zn induced reactive oxygen species (ROS) production and malondialdehyde (MDA) content. Transcriptomic analysis showed that 303, 712, and 2534 differentially expressed ? genes (DEGs) were identified after exposure upon 0.01, 0.1, and 1 mg/L Zn, respectively. Most of these DEGs were downregulated implying that gene expressions were largely inhibited by Zn, which might lead to impairments ? of biological functions. In addition, 10 DEGs involved in oxidative stress, immune response, apoptosis, etc. were selected for qRT-PCR validation ? and showed consistent results with the RNA-seq data. Additionally, the expression level of 12 selected genes based on the transcriptomic data was monitored at different Zn concentrations in the hepatopancreas. Results revealed that? L. vannamei ?might? resist ?the toxicity of Zn at the transcriptomic level ?by changing expressions of immune-, detoxification-related genes, ?etc. These results provide novel insight into the molecular toxicology of Zn on shrimp and a good basis for assessing the environmental risk of Zn. ? 2022, The Authors. All rights reserved.