文献类型：期刊文献

英文题名：Cloning, identification and functional analysis of a beta-catenin homologue from Pacific white shrimp, Litopenaeus vannamei

作者：Zhang, Shuang[1];Shi, Lili[1];Lu, Kai[2,3,4];Li, Haoyang[2,3,4];Wang, Sheng[2,3,4];He, Jianguo[2,3,4,5];Li, Chaozheng[2,3,4,5,6]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China;[2]Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, MOE Key Lab Aquat Product Safety, Guangzhou 510275, Guangdong, Peoples R China;[3]Sun Yat Sen Univ, Inst Aquat Econ Anim, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China;[4]Guangdong Prov Key Lab Marine Resources & Coastal, Guangzhou, Guangdong, Peoples R China;[5]Sun Yat Sen Univ, Sch Marine Sci, Guangzhou 510275, Guangdong, Peoples R China;[6]South China Sea Resource Exploitat & Protect Coll, Xiamen, Peoples R China

年份：2016

卷号：54

起止页码：411

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000378451700048)、、WOS

基金：This research was supported by National Natural Science Foundation of China 31402321 and U1131002; Chinese Agriculture Research system (CARS-47); The PhD Start-up Fund of Natural Science Foundation of Guangdong Province, China (2014A030310184); Youth Innovation Talent Project of Ordinary university in Guangdong Province, China (2014KQNCX079); Special support program for Outstanding Young Teachers of Guangdong Ocean University (HDYQ2015004). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：beta-Catenin; Litopenaeus vannamei; Wnt pathway; WSSV; Vibrio parahaemolyticus; Antimicrobial peptides (AMPs)

外文摘要：Wnt signaling is known to control multiple of cellular processes such as cell differentiation, communication, apoptosis and proliferation, and is also reported to play a role during microbial infection. beta-catenin is a key regulator of the Wnt signaling cascade. In the present study, we cloned and identified a beta-catenin homologue from Litopenaeus vannamei termed Lv beta-catenin. The full-length of Lv beta-catenin transcript was 2797 bp in length within a 2451 bp open reading frame (ORF) that encoded a protein of 816 amino acids. Lv beta-catenin protein was comprised of several characteristic domains such as an N-terminal region of GSK-beta consensus phosphorylation site and Coed coil section, a central region of 12 continuous Armadillo/beta-Catenin-like repeat (ARM) domains and a C-terminal region. Real-time PCR showed Lv beta-catenin expression was responsive to Vibrio parahaemolyticus and white spot syndrome virus (WSSV) infection. Dual-reporter analysis showed that over-expression of Lv beta-catenin could induce activation of the promoter activities of several antimicrobial peptides (AMPs) such as shrimp PEN4, suggesting that Lv beta-catenin could play a role in regulating the production of AMPs. Knockdown of Lv beta-catenin enhanced the sensitivity of shrimps to V. parahaemolyticus and WSSV challenge, suggesting Lv beta-catenin could play a positive role against bacterial and viral pathogens. In summary, the results presented in this study provided some insights into the function of Wnt/beta-catenin of shrimp in regulating AMPs and the host defense against invading pathogens. (C) 2016 Elsevier Ltd. All rights reserved.