文献类型：期刊文献

英文题名：Integrated transcriptomic and metabolomic analysis reveals the response of pearl oyster (Pinctada fucata martensii) to long-term hypoxia

作者：Yang, Chuangye[1];Wu, Hailing[1];Chen, Jiayi[1];Liao, Yongshan[2,3];Mkuye, Robert[1];Deng, Yuewen[1,2,3,4,5];Du, Xiaodong[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Sci & Innovat Ctr Pearl Culture, Zhanjiang 524088, Peoples R China;[3]Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang 524088, Peoples R China;[4]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[5]Guangdong Marine Ecol Early Warning & Monitoring L, Zhanjiang 524088, Peoples R China

年份：2023

卷号：191

外文期刊名：MARINE ENVIRONMENTAL RESEARCH

收录：SCI-EXPANDED(收录号：WOS:001062985900001)、、EI(收录号：20233314566737)、Scopus(收录号：2-s2.0-85167802520)、WOS

基金：This work was supported by the Open Fund of Key Laboratory of Tropical Marine Bio-resources and Ecology of Chinese Academy of Science and Guangdong Provincial Key Laboratory of Applied Marine Biology (Grant No. 2021011002), National Natural Science Foundation of China (Grant No. 32102817), and Department of Education of Guangdong Province (Grant No. 2020ZDZX1045 and 2021KCXTD026), the earmarked fund for CARS-49, Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (Grant No. 2023KJ146), and the program for scientific research start-up funds of Guangdong Ocean University (060302022304). We thank TopEdit (www.topeditsci.com) for linguistic assistance during the preparation of this manuscript. We are very grateful to Marine Pearl Science and Technology Backyard in Leizhou of Guangdong for providing our experimental site. Metabolomics analysis was assisted by Biotree Biotech Co., Ltd. (Shanghai, China).

语种：英文

外文关键词：Hypoxic stress; Transcriptome; Metabolome; Pearl oyster

外文摘要：The frequency at which organisms are exposed to hypoxic conditions in aquatic environments is increasing due to coastal eutrophication and global warming. To reveal the effects of long-term hypoxic stress on metabolic changes of pearl oyster, commonly known as Pinctada (Pinctada fucata martensii), the present study performed the integrated analysis of transcriptomics and metabolomics to investigate the global changes of genes and metabolites following 25 days hypoxia challenge. Transcriptome analysis detected 1108 differentially expressed genes (DEGs) between the control group and the hypoxia group. The gene ontology (GO) analysis of DEGs revealed that they are significantly enriched in functions such as "microtubule-based process", "histone (H3-K4, H3-K27, and H4-K20) trimethylation", "histone H4 acetylation", "kinesin complex", and "ATPase activity", and KEGG pathway functions, such as "DNA replication", "Apoptosis", and "MAPK signaling pathways". Metabolome analysis identified 68 significantly different metabolites from all identified metabolites, and associated with 25 metabolic pathways between the control and hypoxia groups. These pathways included aminoacyl-tRNA biosynthesis, arginine and proline metabolism, and phenylalanine metabolism. Our integrated analysis suggested that pearl oysters were subject to oxidative stress, apoptosis, immune inhibition, and neuronal excitability reduction under long-term hypoxic conditions. We also found a remarkable depression in a variety of biological functions under long-term hypoxia, including metabolic rates, biomineralization activities, and the repression of reorganization of the cytoskeleton and cell metabolism. These findings provide a basis for elucidating the mechanisms used by marine bivalves to cope with long-term hypoxic stress.