文献类型：期刊文献

中文题名：水产品中麻痹性贝类毒素(PSTs)检测技术的研究进展

英文题名：Developments in Paralytic Shellfish Toxin(PST)-detection Techniques

作者：杨锡洪[1,2];张丽娜[1];解万翠[1,3];章超桦[1]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点试验室,水产品深加工广东普通高等学校重点试验室,国家贝类加工技术研发分中心(湛江),广东湛江524088;[2]青岛科技大学化学与分子工程学院,山东青岛266042;[3]青岛科技大学化工学院,山东青岛266042

年份：2016

卷号：32

期号：12

起止页码：366

中文期刊名：现代食品科技

外文期刊名：Modern Food Science and Technology

收录：CSTPCD、、北大核心2014、Scopus、北大核心

基金：国家自然科学基金资助项目(31271938);现代农业产业技术体系专项(GARS-48)

语种：中文

中文关键词：麻痹性贝类毒素;性质;生物法;仪器分析;生化分析

外文关键词：paralytic shellfish toxins;characterization;biological methods;instrumental analysis technologies;biochemical testing technologies

中文摘要：麻痹性贝类毒素是一种分布范围广及危害较大的赤潮毒素。可经食物链的富集、传递作用,引发人体麻痹性中毒,大量发生的中毒事件,对人类健康和经济构成了严重威胁。目前,麻痹性贝类毒素常用检测技术主要是小鼠生物法、高效液相色谱法和酶联免疫试剂盒测试法,这些检测方法均有各自的优势,但麻痹性贝类毒素成分多且复杂、结构特殊,毒性又较强,这使得其监管检测较为困难,亟待建立快速简便、灵敏度高、特异性强的分析检测方法。本文基于麻痹性贝类毒素的基本性质,依据检测原理的不同论述了其生物检测技术、仪器分析技术和生化测试技术,并对各类技术的特点进行分析,提出建设性的意见,最后展望了未来麻痹性贝类毒素检测技术的发展趋势,以期为麻痹性贝类毒素检测监管提供借鉴。

外文摘要：Paralytic shellfish poisoning, caused by the ingestion of shellfish contaminated with paralytic shellfish toxins（PSTs）, has been reported in many countries. These poisoning incidents have resulted in losses to local economies due to a negative impact on shellfish harvests. Currently, the mouse bioassay（MBA）, high performance liquid chromatography（HPLC）, and enzyme-linked immunosorbent assay（ELISA） are widely used to detect PSTs. Although these techniques have many advantages, high toxicity and the presence of many complex components and special structures makes the analysis of this group of compounds especially challenging. A rapid, simple, effective, and specific method needs to be designed. Biological methods, techniques for instrumental analysis, and biochemical tests for the detection of PSTs in shellfish, cyanobacteria, and contaminated water are summarized in this paper, and their advantages and disadvantages for particular applications are discussed. Future studies that will contribute to their improvement are also discussed.