文献类型：期刊文献

英文题名：Prevalence of virulence genes and antibiotic susceptibility of Bacillus used in commercial aquaculture probiotics in China

作者：Anokyewaa, Melody Abena[1,2,3,5];Amoah, Kwaku[1,4];Li, Yuan[1,2,3];Lu, Yishan[1,2,3];Kuebutornye, Felix K. A.[1,2,3];Asiedu, Berchie[5];Seidu, Iddrisu[5,6]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Guangdong, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518120, Guangdong, Peoples R China;[4]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Anim Nutr & Feed, Zhanjiang 524088, Guangdong, Peoples R China;[5]Univ Energy & Nat Resources, Dept Fisheries & Water Resources, Sunyani, Ghana;[6]Kocaeli Univ, Dept Fisheries Sci, Kocaeli, Turkey

年份：2021

卷号：21

外文期刊名：AQUACULTURE REPORTS

收录：SCI-EXPANDED(收录号：WOS:000701693500005)、、Scopus(收录号：2-s2.0-85110200924)、WOS

基金：National Key Research and Development Project2020YFD0900201. Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams (2019KJ141)

语种：英文

外文关键词：Commercial probiotics; Bacillus; Safety; Virulence genes; Enterotoxin; Antibiotic resistance

外文摘要：Disease is a significant constraint faced by aquaculture, and its prevention and control bring together a hub of recent research. Several resources and resorts have been applied to prevent diseases in aquaculture. Probiotics are known to be beneficial natural derivatives that have several benefits in aquaculture. Currently, several commercial probiotics are used in the aquaculture industry that contains one or more live microorganisms. In the aquaculture industry, Bacillus species is one of the most widely used probiotic organisms. They are considered distinctive and are found to be natural members of the gut microbiota of some fish species. The safety of beneficial microorganisms is essential since some of these organisms are reported to harbor traits that might be transferable to their hosts. In this study, the safety of some Bacillus-based commercial probiotics used in aquaculture in terms of virulence and drug resistance were assessed. Commercial Bacillus species after isolation were screened for the presence of virulence genes (nheA, nheB, nheC, hblA, hblC, hblD, cytK, and entFM) and one emetic gene (ces), as well as their resistance to some antibiotics. Most isolates did not possess any of the virulence genes assessed. Nonetheless, three isolates harbored the nheABC and entFM enterotoxin genes, while two had the hblA, hblC, hblD, cytK genes. None of the isolates possessed the ces emetic gene. Antibiotic resistance assessment revealed most of the isolates to be resistant to beta-lactam antibiotics, including penicillin, ampicillin, oxacillin, cefuroxime, and ceftriaxone, and also to minocycline.