文献类型：期刊文献

中文题名：日粮中铜胁迫对吉富罗非鱼幼鱼抗氧化、脂质过氧化及肝脏组织结构的影响

英文题名：Effects of Dietary Copper Stress on Antioxidation,Lipid Peroxidation and Tissue Structure of Liver in Juvenile GIFT Tilapia( Oreochromis niloticus)

作者：王文龙[1];崔欣[1];安立龙[1];效梅[2];高涵[3];李成成[1];邢星[4];李延伟[1];许英梅[1]

机构：[1]广东海洋大学动物科学系,湛江524088;[2]广东海洋大学动物医学系,湛江524088;[3]中国药科大学药物制剂系,南京211198;[4]中华人民共和国钦州保税港区出入境检验检疫局,钦州535008

年份：2015

卷号：10

期号：2

起止页码：266

中文期刊名：生态毒理学报

外文期刊名：Asian Journal of Ecotoxicology

收录：CSTPCD、、北大核心2014、CSCD2015_2016、北大核心、CSCD

基金：广东省科技计划项目(2010B090400376);国家级大学生创新创业训练计划项目(201310566005)

语种：中文

中文关键词：日粮中铜胁迫;吉富罗非鱼幼鱼;肝脏病变;抗氧化;脂质过氧化;浊肿变性

外文关键词：dietary copper stress; GIFT tilapia; pathological changes in liver; antioxidation; lipid peroxidation;cloudy swelling degeneration

中文摘要：吉富罗非鱼是我国南方沿海地区水产养殖中的主要经济鱼类之一,但近年来,随着我国城镇化和工业化进程的推进,罗非鱼养殖面临着前所未有的铜富集的挑战。为探明日粮中铜胁迫对吉富罗非鱼幼鱼抗氧化系统和肝脏组织结构的影响,将1 080条罗非鱼幼鱼暴露于6个浓度梯度(0、3、30、300、1 000、3 000 mg·kg-1)的高铜日粮中,通过60 d的暴露试验,实时测定罗非鱼血清与肝脏抗氧化能力,监测肝脏病理变化。结果表明,在本试验条件下,罗非鱼幼鱼血清和肝脏中MDA的含量随日粮中铜含量的增加和胁迫时间的延长显著升高,而SOD、GSH-PX和Cu Zn-SOD的活性表现出先升高后降低的趋势;各组间肝脏表现出不同程度的病变,主要是浊肿变性和脂肪变性,且第Ⅲ、Ⅳ、Ⅴ组肝脏病变严重。综上,日粮中铜胁迫对吉富罗非鱼幼鱼的抗氧化机能有较明显的抑制作用,长时间的暴露能严重损伤其肝脏的组织结构,因此,建议吉富罗非鱼幼鱼日粮中铜的实际含量应控制在42.36 mg·kg-1以下。

外文摘要：GIFT tilapia is an important economic species in aquaculture production in coastal regions in south of China, where is now facing an increasing challenge from Cu contamination with accelerated urbanization and industrialization. In order to evaluate the effects of dietary Cu stress on antioxidant activity and liver structure of juvenile GIFT tilapia, a total of 1 080 fishes were fed with 6 different concentrations of Cu in diets （0, 3, 30,300, 1 000, 3 000 mg.kg-1） for 60 days. Antioxidation of serum and liver was measured, and the pathological changes of liver were observed. The results showed that the concentration of MDA in serum and liver increased significantly with exposure time and the increase of Cu accumulation in diets. Activities of SOD, CuZn-SOD and GSH-PX showed a down trend afler a rise. Meanwhile, cloudy swelling and fatty degeneration in ceils of liver was observed, especially pathological changes of liver aggravated when Cu concentration was increased to groupⅢ , Ⅳ and V. It could be concluded that dietary Cu stress depressed the antioxidant activity and damaged the liver oftilapia. Therefore, we suggest that Cu concentration in diets for juvenile tilapia should not be more than 4236 mg.kg-1.