文献类型：期刊文献

英文题名：Structural and functional characterization of neuromedin S in the teleost fish, zebrafish (Danio rerio)

作者：Chen, Huapu[1,2];Huang, Hongxin[2];Chen, Xinggui[2];Deng, Siping[1];Zhu, Chunhua[1];Huang, Hai[3];Li, Guangli[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Guangdong Med Coll, Affiliated Hosp, Zhanjiang 524001, Guangdong, Peoples R China;[3]Qiongzhou Univ, Sanya 572022, Hainan, Peoples R China

年份：2016

卷号：191

起止页码：76

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000366441500009)、、WOS

基金：This work is supported by the Guangdong Provincial Natural Science Fund (no. S2013040013257), Guangdong Province Marine Fishery Science and Technology Promotion Project (no. A201408A06), the Hainan Province Marine Science and Technology (no. XH201401) and Hainan International Science and Technology Cooperation Projects (no. KJHZ2015-08).

语种：英文

外文关键词：Feeding; Neuroendocrine regulation; Neuromedin S; Zebrafish

外文摘要：Neuromedin S (NMS) has been demonstrated to have important roles in many vertebrate physiological processes. However, the function of NMS in teleost fishes remains unclear. We explored the physiological roles of the NMS gene in the zebrafish model. An NMS cDNA was cloned from zebrafish brain tissue, and the full-length cDNA sequence was 521 bp in length and encoded a precursor of 110 amino acid residues. Interestingly, fish prepro-NMS is predicted to generate a short 34-residue peptide, designated as NMS-related peptide (NMSRP). Zebrafish prepro-NMS does not contain the NMS peptide which is found in the NMS precursors of mammals, and just retains the MNSRP peptide. A multiple-species sequence alignment showed that NMSRPs are conserved among the other sampled vertebrates. Zebrafish NMS mRNA was detected by RT-PCR revealing a tissue-specific distribution with high levels of expression in the brain, spleen, ovary, pituitary, and muscle. Furthermore, the locations of NMS-expressing cells in the zebrafish brain were detected by in situ hybridization in the parvocellular preoptic nucleus (PPa), the ventral zone of the periventricular hypothalamus (Hv), and lateral hypothalamic nucleus (LH). The levels of NMS mRNA in the hypothalamus were significantly increased after three days of food deprivation. Administration of zebrafish NMSRP by intraperitoneal injection significantly promoted the expression of neuropeptide Y (NPY) and orexin, suggesting an orexigenic role for NMSRP in zebrafish. The present study offers a new understanding of the NMS gene in vertebrates and increases our knowledge of the neuroendocrine regulation of feeding. (C) 2015 Elsevier Inc All rights reserved.