文献类型：期刊文献

英文题名：Effect of pure microcystin-LR on activity and transcript level of immune-related enzymes in the white shrimp (Litopenaeus vannamei)

作者：Chen, Yanyan[1,2,3];Huang, Xianghu[1,2,3];Wang, Jianzhu[4];Li, Changling[1,2,3]

机构：[1]Guangdong Ocean Univ, Fishery Coll, Dept Aquaculture, Zhanjiang 524088, Guangdong, Peoples R China;[2]Engn Technol Res Ctr Algae Breeding & Applicat, Zhanjiang 524088, Guangdong, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Res Inst, Shenzhen 518108, Peoples R China;[4]Three Gorges Univ, Collaborat Innovat Ctr Geohazards & Ecoenvironm G, Yichang 443002, Peoples R China

年份：2017

卷号：26

期号：5

起止页码：702

外文期刊名：ECOTOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:000403461400012)、、Scopus(收录号：2-s2.0-85018403240)、WOS

基金：This study was supported by Guangdong Special Marine fishery Science and Technology Promotion Project (A201308B10) and Guangdong Special Marine Fishery Science and Technology and Industrial Development Project (A201508B08). The authors thank Natalie Defraene for English editing, and an anonymous reviewer for critical comments.

语种：英文

外文关键词：Microcystin-LR; Immune-related enzymes; Transcript level; Litopenaeus vannamei

外文摘要：Microcystins (MCs) in freshwater and marine waters released by toxin-producing cyanobacteria have negative impacts to the aquatic environment. This study aimed to investigate the effect of pure microcystin-LR on activity and transcript level of immune-related enzymes in the white shrimp Litopenaeus vannamei. After exposed to varying concentrations of pure microcystin-LR (MC-LR) for 30 days, the activity of superoxide dismutase (SOD), lysozyme (LZM), glutathione peroxidase (GPx), peroxidase (POD), acid phosphatase (ACP), alkaline phosphatase (AKP) and transcript level of cMn-sod, lzm, gpx were investigated in the hepatopancreas of white shrimp (L. vannamei). Immune-related enzyme activities responded differently to MC-LR exposure. SOD, GPx, and POD activity in the hepatopancreas were activated in a concentration-dependent manner while LZM activity was significantly inhibited in the treatment groups. ACP and AKP activity showed an increase, followed by a decrease. The transcript levels of cMn-sod, lzm, and gpx were consistent with changes in their encoding enzyme activity. These results demonstrated that sub-chronical exposure to MC-LR induced the alteration of immune-related enzymes and corresponding genes in the hepatopancreas, which may help explain the presence of detoxification mechanisms in crustaceans and how they were protected from MC-LR stress for a long period of time.