文献类型：期刊文献

英文题名：The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells

作者：Ibtisham, F.[1];Zhao, Y.[1];Nawab, A.[1];Liguang, H.[1];Wu, J.[1];Xiao, M.[1];Zhao, Z.[1];An, L.[1]

机构：[1]Guangdong Ocean Univ, Agr Coll, Zhanjiang, Guangdong, Peoples R China

年份：2018

卷号：20

期号：3

起止页码：463

外文期刊名：BRAZILIAN JOURNAL OF POULTRY SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000446302300009)、、Scopus(收录号：2-s2.0-85054707514)、WOS

基金：This work was supported by the Science and Technology Planning Project of Guangdong Province, China (2010 B090400376).

语种：英文

外文关键词：Apoptosis; Chicken embryonic fibroblast; Cell proliferation; Heat stress; Oxidative stress

外文摘要：The effects of oxidative stress induced by high temperature on the cell viability, proliferation, apoptosis and oxidative status of chicken embryonic fibroblasts (CEF) were analyzed. The viability, proliferation, apoptotic and anti-oxidative status were measured after incubating CEF at the temperatures of 37 degrees C (control) and 40-44 degrees C (experimental groups) for 6,12 and 24 hours. The results showed that at high temperature (42-43 degrees C), the viability of CEF cells decreased after 6, 12 and 24 h of incubation, but the difference was significant only at 43 degrees C. Cell proliferation was significantly reduced at 44 degrees C/6h. The apoptotic rate of CEF cells was increased following heat treatments in a time-dependent manner. ROS formation increased with increasing temperature, but the difference was only significant at 44 degrees C/6,12h. Heat stress did not significantly affect the superoxide dismutase (SOD) activity. CAT activity was significantly decreased at 43 degrees C/24h and 44 degrees C/12 and 24h. Malondialdehyde (MDA) formation was significantly increased at 43 degrees C/12h and 44 degrees C/12 and 24h. In conclusion, heat stress induced the oxidative stress, decreasing the viability, proliferation and anti-oxidative response of CEF cells.