文献类型：期刊文献

英文题名：An enzyme free fluorescence resonance transfer strategy based on hybrid chain reaction and triplex DNA for Vibrio parahaemolyticus

作者：Tan, Xiao-Hui[1];Li, Yu-Bin[1];Liao, Yan[2];Liu, Hua-Zhong[1]

机构：[1]Guangdong Ocean Univ, Coll Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China

年份：2020

卷号：10

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：SCI-EXPANDED(收录号：WOS:000596329600066)、、Scopus(收录号：2-s2.0-85096637382)、WOS

基金：This work is supported by the foundation and applied foundation research Joint-Youth Fund project of Guangdong province, China (No. 2019A1515110648) and the Science and Technology Project on Special Fund for Public Welfare Research and Ability Construction of Guangdong Province, China (No. 2017A010105010).

语种：英文

外文摘要：In this work, an enzyme-free fluorescence resonance energy transfer (FRET) strategy was established for rapid and specific detection of the DNA sequence from Vibrio parahaemolyticus (VP) using hybridization chain reaction (HCR) amplification and triplex DNA. The triplex forming oligonucleotide (TFO) was labelled with carboxyfluorescein (FAM) as fluorescence donor, and hairpin sequence H1 was labelled by tetramethylrhodamine (TAMRA) as fluorescence receptor. In the present target VP DNA, the hairpin structure of molecular beacon (MB) was opened, the free end was released and hybridized with H1-TAMRA, and the HCR reaction was triggered by the alternate supplementation of H1-TAMRA and H2 to produce the notch double helix analogue. After the addition of TFO-FAM, a triplex structure was formed between HCR products (H1-TAMRA/H2) and TFO-FAM. A close contact between the donor and the receptor resulted in FRET. Under the optimal conditions, the fluorescence quenching value was inversely proportional to the concentration of target VP DNA in the range of 0.1-50 nmol L-1, and the detection limit was 35 pmol L-1.