详细信息
超声波平板法检测溶藻弧菌HY9901生物被膜的研究 被引量:3
Optimization of Ultrasonic-plate Method for Quantitative Detection of Bacterium Vibrio alginolyticus(HY9901) Biofilm
文献类型:期刊文献
中文题名:超声波平板法检测溶藻弧菌HY9901生物被膜的研究
英文题名:Optimization of Ultrasonic-plate Method for Quantitative Detection of Bacterium Vibrio alginolyticus(HY9901) Biofilm
作者:王蓓[1];吴灶和[1];简纪常[1];鲁义善[1];汤菊芬[1];黄郁葱[1]
机构:[1]广东海洋大学水产学院广东省水产经济动物病原生物学及流行病学重点试验室暨广东省高等学校水产经济动物病害控制重点实验室,广东湛江524025
年份:2009
卷号:28
期号:7
起止页码:378
中文期刊名:水产科学
外文期刊名:Fisheries Science
收录:CSTPCD、、北大核心2008、北大核心
基金:广东省科学技术厅社会发展项目(2006A20301003)
语种:中文
中文关键词:生物被膜;溶藻弧菌;超声波平板法;优化条件
外文关键词:biofilm; Vibrio alginolyticus ; ultrasonic-plate method; optimization
中文摘要:以溶藻弧菌为研究对象,对定量检测生物被膜的超声波平板法进行条件优化,对优化的超声波平板法与平板擦拭法进行比较,并采用银染法进行验证。试验结果表明,优化的超声波平板法的最佳条件为:超声波功率120 W,处理14 min,处理温度25℃,优于平板擦拭法。
外文摘要:Biofilms are characterized by widespread and hard to be dete tential risk to aquaculture production and fishery product safety. The ed and eliminated,which are poltrasonic-plate method was optimized to eliminate bacterium Vibrio alginolyticus as a biofilms model strain and compared with the plateswabbing method and then the silver-staining method was used for verification. The results showed that the optimal conditions for ultrasonic-plate method were found to be: ultrasonic power of 120 W, treatment time 14 min and temperature of 25 ℃. The optimized ultrasonic-plate method was superior to the plateswabbing method.
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