文献类型：期刊文献

英文题名：Identification and characterization of circular RNAs in chicken hepatocytes

作者：Zhang, Li[1];Xu, Haidong[1];Wang, Zhang[1];Li, Ting[1];Guo, Jiali[1];Adu-Asiamah, Patricia[1];Leng, Qiying[1];An, Lilong[1];Liu, Manqing[2];Zhang, Xiquan[2];Zhao, Zhihui[1]

机构：[1]Guangdong Ocean Univ, Agr Coll, Zhanjiang 524088, Peoples R China;[2]South China Agr Univ, Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China

年份：2019

卷号：46-47

起止页码：16

外文期刊名：GROWTH HORMONE & IGF RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000479024900003)、、Scopus(收录号：2-s2.0-85065759414)、WOS

基金：This work was supported by the National Natural Science Foundation (31672412), the Guangdong Provincial Natural Science Foundation (2017A030307002), the Open Fund of Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture (Guangzhou), Innovation Training of Guangdong Ocean University (CXXL2018017) and The Key Platform Projects of Innovative Strong School Engineering by Department of Education of Guangdong Province: Efficient Use of Animal Genetic Resources in the Tropics and Healthy Farming (2018302).

语种：英文

外文关键词：Circular RNAs; Hepatocyte; GHR

外文摘要：CircRNAs play important roles in chicken's growth. In this context, we studied the expression profiles of circRNAs between the GHR antisense transcript overexpressed and the control groups of chicken hepatocytes by using the deep RNA-sequencing technique to identify the key circRNAs involved in chicken's growth. A total of 4772 circRNAs were detected in both groups and 92 circRNAs displayed significant differential expression between two groups. Reactome pathway analysis on the parental genes of differential circRNAs indicated that most enriched and meaningful 9 pathways were related to "Mitotic G1-G1/S-M phases", and "FGFR2c ligand binding and activation". Additionally, Five exonic circRNAs were confirmed including circGHR separated from GHR. CircGHR developed in HuaiXiang chicken from d 1 to 5 w of age and was expressed at a higher level in the nucleus than in the cytoplasm. Moreover, our results specify 5 circRNAs target sites for microRNA Let-7b that is expected to target GHR mRNA, signifying their potential role in regulating GHR gene expression in chicken.