文献类型：期刊文献

英文题名：Cloning and mRNA expression of selenium-dependent glutathione peroxidase from the oyster Magallana hongkongensis in response to cadmium exposure and aerial exposure at different temperatures

作者：Chen, Citing[1];Gao, Jialong[1,2,3];Zhang, Chaohua[1,2,3];Qin, Xiaoming[1,2,3];Cao, Wenhong[1,2,3];Zheng, Huina[1,2,3];Lin, Haisheng[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Natl Res & Dev Branch Ctr Shellfish Proc Zhanjiang, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Guangdong Prov Engn Lab Marine Biol Prod, Zhanjiang 524088, Peoples R China;[3]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China

年份：2023

卷号：89

期号：5

起止页码：633

外文期刊名：FISHERIES SCIENCE

收录：SCI-EXPANDED(收录号：WOS:001025586500001)、、WOS

基金：This work was supported by the National Key R & D Program of China (2018YFD0901105), the Natural Science Foundation of Guangdong Province (2017A030310626), the program for scientific research start-up funds of Guangdong Ocean University, and the special fund for the Construction of Modern Agricultural Industrial Technology System (No. CARS-49).

语种：英文

外文关键词：Magallana hongkongensis; Glutathione peroxidase; Gene expression; Cadmium exposure; Aerial exposure at different temperatures

外文摘要：Glutathione peroxidase is an important enzyme in the endogenous antioxidant defense system. In the current study, we cloned the full-length cDNA sequence that encodes a glutathione peroxidase from Magallana hongkongensis (designated as MhGPx), investigated the tissue expression profile of MhGPx mRNA, and studied the time-dependent effects of cadmium exposure and air exposure at two temperatures (4 & DEG;C and 15 & DEG;C) on the mRNA level of MhGPx in different tissues (including gill, adductor, mantle, and digestive gland). The full length of MhGPx was 1306 bp. This gene was found to contain a selenocysteine insertion sequence within the 3 & PRIME; untranslated region, which indicates that the protein it encodes was a selenoprotein. In addition, MhGPx was constitutively expressed in all four selected tissues, and the level of expression in the digestive gland was highest, indicating that MhGPx mRNA expression has tissue-specificity. Under cadmium exposure, mRNA expression was significantly increased on day 1. When exposed to air, the highest MhGPx mRNA expression in the 4 & DEG;C group was lower and appeared later than that of the 15 & DEG;C group. The results of this study contribute to a better understanding of oyster GPx in response to oxidative stress.