文献类型：期刊文献

英文题名：Loop-mediated isothermal amplification method for rapid detection of Vibrio alginolyticus, the causative agent of vibriosis in mariculture fish

作者：Cai, S.H.[1,2]; Lu, Y.S.[1,2]; Wu, Z.-H.[1,2]; Jian, J.C.[1,2]; Wang, B.[1,2]; Huang, Y.C.[1,2]

机构：[1] Fisheries College, Guangdong Ocean University, 40 Jiefang East Rd., Zhanjiang Guangdong 524025, China; [2] Guangdong Provincial, Key Laboratory of Pathogenic Biology, Epidemiology for Aquatic Economic Animals, Zhanjiang, China

年份：2010

卷号：50

期号：5

起止页码：480

外文期刊名：Letters in Applied Microbiology

收录：EI(收录号：20231113724975)、Scopus(收录号：2-s2.0-77950481239)

语种：英文

外文关键词：DNA - Genes - Marine biology - Meats

外文摘要：Aims: The purpose of this study was to develop a loop-mediated isothermal amplification (LAMP) method for the rapid, sensitive and simple detection of Vibrio alginolyticus in mariculture fish. Methods and Results: LAMP primers were designed by targeting the gyrB gene. With Bst DNA polymerase, the target DNA can be clearly amplified for 60 min at 64°C in a simple water bath. The detection sensitivity of the LAMP assay for the detection of V. alginolyticus is about 3·7 × 102 CFU ml-1 (3·7 CFU per reaction). LAMP products could be judged with agar gel or naked eye after the addition of SYBR Green I. There were no cross-reactions with other bacterial strains indicating a high specificity of the LAMP. The LAMP method was applied to detect V. alginolyticus-infected fish tissues effectively. Conclusions: The LAMP established in this study is a simple, sensitive, specific, inexpensive and rapid protocol for the detection of V. alginolyticus. Significance and Impact of the Study: This LAMP method provides an important diagnostic tool for the detection of V. alginolyticus infection both in the laboratory and field. ? 2010 The Authors. Journal compilation ? 2010 The Society for Applied Microbiology.