文献类型：期刊文献

英文题名：Fluorescent carbon dots with a high nitric oxide payload for effective antibacterial activity and bacterial imaging

作者：Liu, Shixin[1];Lv, Kai[1];Chen, Ziheng[1];Li, Chengpeng[2];Chen, Tao[3];Ma, Dong[1]

机构：[1]Jinan Univ, Dept Biomed Engn, Guangdong Prov Engn & Technol Res Ctr Drug Carrie, Key Lab Biomat,Guangdong Higher Educ Inst, Guangzhou 510632, Peoples R China;[2]Guangdong Ocean Univ, Sch Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[3]Foshan Forth Peoples Hosp, Dept Lab Med, Foshan 528211, Peoples R China

年份：2021

卷号：9

期号：19

起止页码：6486

外文期刊名：BIOMATERIALS SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000686259600001)、、EI(收录号：20214110995114)、Scopus(收录号：2-s2.0-85116429948)、WOS

基金：This work was financially supported by the "Dengfeng Plan" High-level Hospital Construction Opening Project of Foshan Fourth People's Hospital (FSSYKF2020004), Science and Technology Program of Guangzhou (202102010108) and the Fundamental Research Funds for the Central Universities (21620102).

语种：英文

外文关键词：Biocompatibility - Carbonization - Bacteria - Carbon - Health risks - Fluorescence

外文摘要：Multidrug resistance of bacteria has led to the invalidation of traditional therapies using antibiotics and has formed a huge threat to human health. Therefore, promising antibacterial therapies are urgently demanded. Nitric oxide (NO) has attracted much attention in the field of antibacterial agents, and novel antibacterial materials based on NO are being developed rapidly. In this work, we first proposed a carbon dot (CDs)-based and NO-releasing platform for antibacterial application. Here, the chitosan-graft-poly(amidoamine) dendrimer (CPA) was used to synthesize fluorescent CDs via one-step hydrothermal carbonization, and CPA-CDs were successfully prepared, followed by loading NO with the formation of N-diazeniumdiolate (NONOate). The resultant CPA-CDs/NONOate displayed 3.5 times the NO content of the CPA copolymer. Due to their stable photoluminescence, the super-resolution bacterial imaging ability of CPA-CDs/NONOate was observed. Moreover, excellent in vitro and in vivo antibacterial effects were demonstrated against Pseudomonas aeruginosa, where bacterial viability and biofilm were significantly reduced. Further, in vivo assays proved the theranostic activity of CPA-CDs/NONOate in curing rats' wounds with serious bacterial infection. Importantly, these NO-releasing CDs possessed outstanding in vivo and in vitro biocompatibilities. This study provided a multifunctional strategy, providing a foundation for fast bacterial detection and precise antibacterial treatments.