文献类型：期刊文献

中文题名：超声辅助虾头自溶制备热滞活性产物的工艺研究

英文题名：Research on process of ultrasound-assisted autolysis of shrimp head to prepare active products with thermal hysteresis activity

作者：韩梅[1];欧阳及锦[1];陈秀娟[1];JULIETH Majura[1];陈忠琴[1,2];高加龙[1,2];郑惠娜[1,2];林海生[1,2];曹文红[1,2]

机构：[1]广东海洋大学食品科技学院,国家贝类加工技术研发分中心(湛江),广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东省海洋生物制品工程实验室,广东湛江524088;[2]海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁大连116034

年份：2024

卷号：50

期号：15

起止页码：196

中文期刊名：食品与发酵工业

外文期刊名：Food and Fermentation Industries

收录：北大核心2023、CSTPCD、、Scopus、CSCD2023_2024、北大核心、CSCD

基金：国家自然科学基金项目(321721631006813)。

语种：中文

中文关键词：超声;虾头自溶;热滞活性;氨基酸组成

外文关键词：ultrasound;autolysis;thermal hysteresis activity;amino acid composition

中文摘要：为探究影响超声辅助虾头自溶制备热滞活性(thermal hysteresis activity,THA)产物的因素,该文以水解度为指标,通过Plackett-Burman筛选因子设计,从7个因素中筛选出了超声时间、超声功率、自溶pH、自溶温度、自溶时间,并进行了单因素试验与正交设计,优化出超声辅助虾头自溶的工艺,并探究了不同时间的产物组成变化与热滞活性的关系。结果表明超声功率300 W、超声15 min、pH 8.0、温度50℃、自溶时间3 h时,自溶效果最好,水解度为35.24%。在最优的工艺下进行自溶曲线的实验,发现自溶2 h的产物活性最高;通过关联性分析发现其THA与水解度和氨基酸组成呈强相关关系。自溶2 h时的THA高达1.4℃,水解度为27.18%,其多肽中的抗冻特征性氨基酸含量之和达58.05%。超声辅助虾头自溶对其自溶有促进作用,有开发新型、安全、高效的热滞活性产物的潜力。

外文摘要：Using the degree of hydrolysis as an index,the Plackett-Burman design was adopted to explore the factors affecting the preparation of thermal hysteresis activity(THA)products by ultrasonic-assisted autolysis of shrimp heads.Ultrasonic time,ultrasonic power,autolysis pH,autolysis temperature,and autolysis time were selected from 7 factors.Single factor test and orthogonal design were carried out to optimize the process of ultrasonic-assisted autolysis of shrimp head,and the relationship between product composition and thermal hysteresis activity at different times was explored.Results showed that the autolysis effect was the best when the ultrasonic power was 300 W,the ultrasonic power was 15 min,the pH was 8.0,the temperature was 50℃,the autolysis time was 3 h,and the hydrolysis degree was 35.24%.The autolysis curve experiment was carried out under the optimal process,and it was found that the product of autolysis for 2 h had the highest activity.Correlation analysis showed that THA was strongly correlated with hydrolysis degree and amino acid composition.When the THA at autolysis for 2 h was as high as 1.4℃,the degree of hydrolysis was 27.18%,and the sum of the characteristic anti-freezing amino acid content in the peptides was 58.05%.Ultrasound-assisted autolysis of shrimp head can promote its autolysis and potentially develop new,safe,and efficient thermal hysteresis active products.