文献类型：期刊文献

英文题名：Comparative Transcriptome Analysis ofLitopenaeus vannameiReveals That Triosephosphate Isomerase-Like Genes Play an Important Role During Decapod Iridescent Virus 1 Infection

作者：Liao, Xuzheng[1];Wang, Chenggui[1];Wang, Bo[1];Qin, Haipeng[1];Hu, Shikang[1];Wang, Ping[2];Sun, Chengbo[1,3,4];Zhang, Shuang[1,5]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China;[2]Hainan Zhongzheng Aquat Sci & Technol Co Ltd, Dongfang, Hainan, Peoples R China;[3]Guangdong Prov Lab Southern Marine Sci & Engn, Zhanjiang, Peoples R China;[4]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[5]Aquat Anim Precis Nutr & High Efficiency Feed Eng, Zhanjiang, Peoples R China

年份：2020

卷号：11

外文期刊名：FRONTIERS IN IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000570531900001)、、WOS

基金：This work was supported by the National Key R&D Program of China (grant no. 2019YFD0900200), the National Natural Science Foundation of China (grant no. 31702377), the Project of 2019 Annual Guangdong Provincial Special Financial Fund (grant no. 2319412525), the Fangchenggang Science and Technology Plan Project (grant no. AD19008017), and the Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (grant no. 2019KJ149).

语种：英文

外文关键词：Litopenaeus vannamei; DIV1; triosephosphate isomerase; transcriptome analysis; RNA interference

外文摘要：Decapod iridescent virus 1 (DIV1) results in severe economic losses in shrimp aquaculture. However, little is known about the physiological effect of DIV1 infection on the host. In this study, we found that the lethal dose 50 of DIV1-infectedLitopenaeus vannameiafter 48, 72, 96, and 156 h were 4.86 x 10(6), 5.07 x 10(5), 2.13 x 10(5), and 2.38 x 10(4)copies/mu g DNA, respectively. In order to investigate the mechanisms of DIV1 infection, a comparative transcriptome analysis of hemocytes fromL. vannamei, infected or not with DIV1, was conducted. The BUSCO analysis showed that the transcriptome was with high completeness (complete single-copy BUSCOs: 57.3%, complete duplicated BUSCOs: 41.1%, fragmentation: 0.8%, missing: 0.8%). A total of 168,854 unigenes were assembled, with an average length of 601 bp. Based on homology searches, Kyoto Encyclopedia of Genes and Genomes (KEGG), gene ontology (GO), and cluster of orthologous groups of proteins (KOG) analysis, 62,270 (36.88%) unigenes were annotated. Among them, 1,112 differentially expressed genes (DEGs) were identified, of which 889 genes were up-regulated and 223 genes were down-regulated after DIV1 infection. These genes were mainly annotated to the major metabolic processes such as fructose and mannose metabolism, carbon metabolism, and inositol phosphate metabolism. Among these metabolic pathways, the triosephosphate isomerase (TPI) family was the most eye-catching DEG as it participates in several metabolic processes. Three types ofTPI, LvTPI-like, LvTPI-Blike, andLvTPI-Blike1, were obtained for gene silencing by RNA interference. The results showed thatLvTPI-likeandLvTPI-Blike1silencing caused a high mortality rate amongL. vannamei. However,LvTPI-likeandLvTPI-Blikesilencing reduced DIV1 replication in DIV1-infectedL. vannamei. All the results indicated thatTPI-likegenes play an important role during DIV1 infection, which provides valuable insight into the infection mechanism of DIV1 in shrimp and may aid in preventing viral diseases in shrimp culture.