文献类型：期刊文献

英文题名：Characterization of the shrimp neuroparsin (MeNPLP): RNAi silencing resulted in inhibition of vitellogenesis

作者：Yang, Shi Ping[1];He, Jian-Guo[2];Sun, Cheng Bo[1];Chan, Siuming Francis[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Guangdong, Peoples R China;[2]Zhongshan Univ, Sch Life Sci, Guangzhou, Guangdong, Peoples R China

年份：2014

卷号：4

起止页码：976

外文期刊名：FEBS OPEN BIO

收录：SCI-EXPANDED(收录号：WOS:000346278200115)、、Scopus(收录号：2-s2.0-84912015016)、WOS

基金：The authors would like to thank S.H.K. Tiu and Y.Y. Lam for the technical works. We also thank Professor Jozef Vanden Broeck for the neuroparsin polyclonal antibody. SFC is supported by a start-up fund from the Fisheries College, Guangdong Ocean University.

语种：英文

外文关键词：Shrimp; Neuroparsin; IGF-like binding protein; RNA interference; Vitellogenin

外文摘要：The full-length Metapenaeus ensis neuroparsin (MeNPLP) cDNA was cloned which encodes a shrimp protein homologous to the insect neuroparsin and vertebrate insulin-like growth factor binding protein (IGFBP). MeNPLP cDNA is 1389 bp in length and the longest open reading frame is 303 bp in length. The first 27 aa are predicted to be the signal peptide and aa 28-101 is the mature peptide with an estimated molecular weight of 7.83 kDa and pI of 5. It shows high amino acid sequence similarity (42-68%) to the neuroparsin of insects and N-terminal end of the IGFBP of vertebrates. The cysteine residues in MeNPLP responsible for disulfide bond formation are conserved as in other neuroparsin-like proteins. The expression level of MeNPLP is the highest in the hepatopancreas, followed by the nerve cord, brain, heart, ovary, and muscle. However, it was not expressed in the testis. Using an insect neuroparsin antibody, MeNPLP could only be detected in the hepatopancreatic tubules, suggesting that MeNPLP may be a secretary product. Although MeNPLP expression was stimulated in the ovary, it was inhibited in the hepatopancreas after treatment with neurotransmitter serotonin (5-HT). In vivo gene silencing of MeNPLP could cause a significant decrease of vitellogenin transcript level in the hepatopancreas and ovary. As a result, a corresponding decrease in vitellogenin protein level was observed in the hemolymph and ovary. In conclusion, this study has provided the first evidence that MeNPLP is involved in the initial stage of ovary maturation in shrimp. (C) 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.