文献类型：期刊文献

英文题名：A Microfluidic Prototype System towards Microalgae Cell Separation, Treatment and Viability Characterization

作者：Wang, Yanjuan[1,2,3];Wang, Junsheng[1,2,4];Zhou, Chen[1,2];Ding, Gege[1,2];Chen, Mengmeng[1,2];Zou, Jiang[1,2];Wang, Ge[1,2];Kang, Yuejun[5];Pan, Xinxiang[6]

机构：[1]Dalian Maritime Univ, Ctr Microfluid & Optoelect Sensing, Dalian 116026, Peoples R China;[2]Dalian Maritime Univ, Coll Informat Sci & Technol, Dalian 116026, Peoples R China;[3]Dalian Jiaotong Univ, Software Technol Inst, Dalian 116028, Peoples R China;[4]Guangdong Ocean Univ, Nav Coll, Zhanjiang 524088, Peoples R China;[5]Southwest Univ, Sch Mat & Energy, Chongqing 400715, Peoples R China;[6]Guangdong Ocean Univ, Coll Elect & Informat Engn, Zhanjiang 524088, Peoples R China

年份：2019

卷号：19

期号：22

外文期刊名：SENSORS

收录：SCI-EXPANDED(收录号：WOS:000503381500119)、、EI(收录号：20194707730554)、Scopus(收录号：2-s2.0-85075287273)、WOS

基金：This research was funded by National Key Research and Development Program of China (2017YFC1404606, 2017YFC1404603), National Natural Science Foundation of China (51779027), LiaoNing Revitalization Talents Program (XLYC1807036), Liaoning Natural Science Foundation (2019-MS-025) and BaiQianWan Talents Program to JunshengWang, the Fundamental Research Funds for the Central Universities (3132019217, 3132019336), Dalian science and technology innovation fund (2018J11CY021).

语种：英文

外文关键词：microfluidic chip; deterministic lateral displacement; concentration gradient generator; single photon detection; chlorophyll fluorescence

外文摘要：There are a huge number, and abundant types, of microalgae in the ocean; and most of them have various values in many fields, such as food, medicine, energy, feed, etc. Therefore, how to identify and separation of microalgae cells quickly and effectively is a prerequisite for the microalgae research and utilization. Herein, we propose a microfluidic system that comprised microalgae cell separation, treatment and viability characterization. Specifically, the microfluidic separation function is based on the principle of deterministic lateral displacement (DLD), which can separate various microalgae species rapidly by their different sizes. Moreover, a concentration gradient generator is designed in this system to automatically produce gradient concentrations of chemical reagents to optimize the chemical treatment of samples. Finally, a single photon counter was used to evaluate the viability of treated microalgae based on laser-induced fluorescence from the intracellular chlorophyll of microalgae. To the best of our knowledge, this is the first laboratory prototype system combining DLD separation, concentration gradient generator and chlorophyll fluorescence detection technology for fast analysis and treatment of microalgae using marine samples. This study may inspire other novel applications of micro-analytical devices for utilization of microalgae resources, marine ecological environment protection and ship ballast water management.