文献类型：期刊文献

中文题名：红鳍笛鲷弧菌病病原的研究

英文题名：Pathogen of Vibriosis of Lutjanus sanguineus

作者：李永芹[1];简纪常[1];吴灶和[1]

机构：[1]湛江海洋大学水产学院,广东省水产经济动物病原生物学及流行病学重点实验室暨广东省教育厅水产经济动物病害控制重点实验室,广东湛江524025

年份：2004

卷号：24

期号：6

起止页码：1

中文期刊名：湛江海洋大学学报

外文期刊名：Journal of Zhanjiang Ocean University

收录：CSTPCD

基金：广东省科技计划重大专项(A3050302);广东省农业攻关项目(2004B20301002);湛江市农业招标项目;湛江市科技兴海项目(0309005)

语种：中文

中文关键词：红鳍笛鲷;弧菌病;病原;溶藻弧菌;细菌鉴定;药敏试验

外文关键词：Lutjanus sanguineus; Vibriosis;pathogen; Vibrio alginolaticus; bacterial identification; drug sensitivity

中文摘要：从广东湛江特呈岛鱼排取患病红鳍笛鲷进行病原分离、纯化,得到一株病原菌Ls 1。该菌 为革兰阴性短杆菌,有运动性,菌落半透明,最适温度28～30℃,需盐生长,氧化酶反应阳性,发酵 葡萄糖不产气,不发酵肌醇,对弧菌抑制剂O/129敏感。经API ID32E细菌鉴定系统及弧菌科细 菌生化鉴定管鉴定,该菌为一株溶藻弧菌(Vibrioaginolyticus);其对红鳍笛鲷的半数致死量为6.32 ×105mL-1。药敏试验结果表明,该菌对阿莫西林、先锋V不敏感,对新生霉素、卡那霉素、多粘菌 素B、四环素中度敏感,而对庆大霉素、氟哌酸、氯霉素、红霉素、链霉素和复方新诺明等有较高的敏 感性。

外文摘要：A strain of pathogenic bacteria Ls-1 was isolated and purified from the sick blood-red snapper (Lutjanus sanguineus)which was maricultured in the cages of Techeng island of Zhanjiang city in Guangdong province. The bacterium shared following characteristics: Gram-negative, short rod, motile, semitransparent bacteria colony, optimal temperature of 28~30 ℃, growth only in medium contained NaCl, oxidase-positive, fermentation of glouse without production of gas, non-fermentation of Inositol, sensitive to the vibriostatic agent O/129.The Ls-1 strain was identified as Vibrio aginolyticus by the data of API-ID32E kit and the Vibrio identification system. The 50% lethal dose of the bacterium to the fish was 6.32×10 5 cfu/mL. The result of drug sensitivities indicated that novobiocin, kanamycin, polymyxin B, tetracycline, chloramphenicol, norfloxacin, chloromyctin, ciprofloxacin, streptomycin and compound sulfamethoxazolecould inhibit the growth of strain Ls-1 to the different extents, but amoxicillin and cefazolinSodium couldn’t do.