文献类型：期刊文献

中文题名：对虾酚氧化酶微量测定体系的建立与评价

英文题名：Establishment and Assessment of Microdetermination System for Monitoring the Phenoloxidase Activity in Shrimp

作者：徐德峰[1];李彩虹[2];孙力军[1];王雅玲[1];叶日英[1];廖建萌[3]

机构：[1]广东省水产品加工与安全重点实验室广东海洋大学食品科技学院;[2]广东医学院生物化学与分子生物学教研室;[3]国家海产品质量监督检验中心(湛江)

年份：2015

卷号：0

期号：2

起止页码：174

中文期刊名：中国食品学报

外文期刊名：Journal of Chinese Institute of Food Science and Technology

收录：CSTPCD、、北大核心2014、EI(收录号：20151600764715)、CSCD2015_2016、Scopus(收录号：2-s2.0-84927734325)、北大核心、CSCD

基金：国家自然科学基金项目(31201309;31171634)

语种：中文

中文关键词：对虾;酚氧化酶;微量测定体系

外文关键词：shrimp; phenoloxidase; microdetermination system

中文摘要：快速跟踪对虾保藏过程中的酚氧化酶活力变化,对于改善对虾感官品质和提高产业经济价值具有重要意义。基于酶促反应动力学原理,通过考察反应时间及底物浓度对酶活的影响,建立微量测定体系,并在测定准确度、速度和试剂消耗方面与常量法作对比。结果表明,当反应时间2 min、底物与酶液体积比3∶2时可建立微量测定体系。与常量法相比,微量法在检测酶活性方面无显著性差异,且检测速度是常量法的2倍,试剂消耗量仅为常量法的1/20。微量法具有准确性高,试剂用量少,重复性好,方法简单、快捷等优点。

外文摘要：It is the great significance for improving the sensory quality and economic value of shrimp industry by real-time and accurately monitoring the profile of phenoloxidase activity during storage. Based on the enzyme kinetics,the effects of time course and substrate concentration on the velocity were investigated and the microdetermination system was thus established. And its assessment in accuracy, velocity and reagent consuming was made by comparing with the macrodetermination system. The results showed that microdetermination can be reliably established when the reaction time and the volume rate of substrate to crude enzyme sample were 2 min and 3 ∶ 2, respectively. Compared with the macrodetermination system, this mcirosystem had no significant difference in determination of phenoloxidase activity, but exhibited double speed in velocity and amounted to the volume of 1/20 in reagent consuming. With the advantages of high accuracy, little reagent consuming, excellent repeatability and simplicity, it can be conclude that this microdetermination system will found wide application in phenoloxidase activity monitoring.