文献类型：期刊文献

英文题名：Structural characterization and anticancer enhancement of selenized squid ink glycosaminoglycan nanoparticles

作者：Gu, Yipeng[1,2];Yang, Xiaomei[1];Yang, Ling[1];Yao, Siqi[1];Liu, Huazhong[2]

机构：[1]Hezhou Univ, Inst Food Sci & Technol, Guangxi Key Lab Hlth Care Food Sci & Technol, Hezhou 542899, Peoples R China;[2]Guangdong Ocean Univ, Coll Chem & Environm Sci, Zhanjiang 524088, Peoples R China

年份：2026

卷号：366

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:001769096200001)、、EI(收录号：20262020703980)、Scopus(收录号：2-s2.0-105038631860)、WOS

基金：This work was funded by the General Project of the Natural Science Foundation of Guangxi (2025GXNSFAA069861 & 2025GXNSFAA069190) , the Guangxi Science and Technology Base and Talent Special Project (Guike AD23026036) , the Guangdong Basic and Applied Basic Research Foundation (No. 2022A1515012091) , and the Special Project in Key Fields of Guangdong Universities (No. 2022ZDZX2025) .

语种：英文

外文关键词：Selenized polysaccharide; Structural characterization; Anticancer activity

外文摘要：Squid ink polysaccharide 4 (SIP4), a marine-derived sulfated glycosaminoglycan with favorable biocompatibility and coordination capacity, was employed as a functional template and stabilizing matrix for the synthesis of selenized nanoparticles (SIP4-SeNPs). The optimal SIP4 concentration (0.4 mg/mL) yielded nanoparticles with the highest selenium incorporation (23.88%, ICP-MS), smallest particle size (130.57 nm), and a negative zeta potential (-24.23 mV). Spectroscopic and microscopic analyses (UV-Vis, FT-IR, SEM, DSC, TGA, XRD) confirmed selenium-induced structural reorganization and the formation of uniform, semi-crystalline SIP4-SeNPs stabilized by hydroxyl, carboxyl, and sulfate groups. The nanoparticles exhibited improved stability at 4 degrees C for up to 14 days. Biological evaluations showed that SIP4-SeNPs significantly enhanced antiproliferative activity against MDA-MB-231 cells with a markedly lower IC50 than SIP4, while maintaining low cytotoxicity toward MCF-10 A cells. Apoptosis analysis and ROS detection indicated that SIP4-SeNPs promote cancer cell death through oxidative stress-mediated pathways. This study demonstrates that SIP4-based selenium nanoparticles are promising candidates for breast cancer therapy with improved efficacy and safety.