文献类型：期刊文献

中文题名：奶牛乳腺上皮细胞中Bta-miR-877靶基因的初步验证

英文题名：Validation of Bta-miR-877 target genes in bovine mammary epithelial cells

作者：杨雨薇[2];房希碧[2];于海滨[2];姜平[2];杨润军[2];赵志辉[1,2]

机构：[1]广东海洋大学农学院,广东湛江524088;[2]吉林大学动物科学学院,吉林长春130062

年份：2018

卷号：38

期号：4

起止页码：809

中文期刊名：中国兽医学报

外文期刊名：Chinese Journal of Veterinary Science

收录：CSTPCD、、CSCD_E2017_2018、北大核心2017、北大核心、CSCD

基金：国家自然科学基金资助项目(31372278,31672389);国家“863”计划资助项目(2013AA102505);吉林省省级产业技术研究和发展资助项目(2016C032);吉林省科技发展计划资助项目(20170519014JH)

语种：中文

中文关键词：奶牛乳腺上皮细胞;miR-877;edn1;ptgis

外文关键词：mammary epithelial cells of dairy cows; miR-877 ;edn1;ptgis

中文摘要：利用高通量测序的方法筛选出在高脂奶牛和低脂奶牛中差异表达的miR-877和其预测的靶基因。通过生物信息学方法预测出edn1、ptgis可能是miR877的靶基因，之后通过荧光定量检测miR877以及其靶基因的表达量。结果显示，转染miR877mimics组miR-877的相对表达量显著高于转染miR-877inhibitor组；靶基因的相对表达量，转染miR877mimics组的ednl、ptgis基因表达水平均显著低于转染miR-877inhibitor组（P〈0．01）。经Graph—PadPrism6分析显示，edn1、ptgis基因的相对表达量与miR-877的表达量呈负相关，初步验证edn、ptgis是miR-877的靶基因。本试验为深入了解miR-877在乳脂质代谢调节中的作用提供依据，从而为生产实践提供一定的理论基础。

外文摘要：High-throughput sequencing was used to screen the ditterentially expression if miR-877 and predict its target genes in high-fat and low-fat dairy cows. ednl and ptgis might be the target genes of miR-877 using bioinformatics method analysis,then the expression of miR-877 and its tar- get genes were detected by qPCR. The results showed that the expression of miR-877 was signifi- cantly higher in miR-877 mimics group than miR-877 inhibitor group. The expression levels of ednl and ptgis genes were significantly lower in cells those of transfected miR-877 mimics than miR-877 inhibitor group（P〈0.01）. The relative expression of ednl and ptgis genes were nega- tively correlated with the expression level of miR-877 by Graph Pad Prism6 analysis. It was pre- liminarily verified that edn and ptgis were the target genes of miR-877. It provided a theoretical basis and guidance for production practice in the regulation of lipid metabolism.