文献类型：期刊文献

英文题名：Chitosan-Based Thermo-Sensitive Hydrogel Loading Oyster Peptides for Hemostasis Application

作者：Zhang, Dongying[1,2];Hu, Zhang[1];Zhang, Lingyu[1];Lu, Sitong[1];Liang, Fengyan[1];Li, Sidong[1]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhanjian, Zhanjiang 524088, Peoples R China

年份：2020

卷号：13

期号：21

起止页码：1

外文期刊名：MATERIALS

收录：SCI-EXPANDED(收录号：WOS:000589256900001)、、EI(收录号：20204609498527)、Scopus(收录号：2-s2.0-85095963101)、WOS

基金：The authors gratefully acknowledge the financial support by the Guangdong Provincial Natural Science Foundation of China (2016A030308009), Project of Science and Technology Plan of Guangdong Province (2015A020216019), Project of Science and Technology Plan of Zhanjiang (2019A01017 and 2020A01026), Project of Enhancing School with Innovation of Guangdong Ocean University (2017KTSCX090), and Postgraduate Education Innovation Program of Guangdong Ocean University (201926 and 201927).

语种：英文

外文关键词：chitosan; oyster peptides; hydrogel; hemostasis

外文摘要：Uncontrolled massive hemorrhage is one of the principal causes of death in trauma emergencies. By using catechol-modified chitosan (CS-C) as the matrix material and beta glycerol phosphate (beta-GP) as a thermo-sensitive agent, chitosan-based thermo-sensitive hydrogel loading oyster peptides (CS-C/OP/beta-GP) were prepared at physiological temperature. The hemostatic performance of CS-C/OP/beta-GP hydrogel was tested in vivo and in vitro, and its biological safety was evaluated. The results showed that the in vitro coagulation time and blood coagulation index of CS-C/OP/beta-GP hydrogel were better than those of a commercial gelatin sponge. Notably, compared with the gelatin sponge, CS-C/OP/beta-GP hydrogel showed that the platelet adhesion and erythrocyte adsorption rates were 38.98% and 95.87% higher, respectively. Additionally, the hemostasis time in mouse liver injury was shortened by 19.5%, and the mass of blood loss in the mouse tail amputation model was reduced by 18.9%. The safety evaluation results demonstrated that CS-C/OP/beta-GP had no cytotoxicity to L929 cells, and the hemolysis rates were less than 5% within 1 mg/mL, suggesting good biocompatibility. In conclusion, our results indicate that CS-C/OP/beta-GP is expected to be a promising dressing in the field of medical hemostasis.