文献类型：期刊文献

中文题名：环糊精功能化修饰纳米银负载姜黄素的制备及其抗肿瘤活性

英文题名：Preparation and Anticancer Activity of Cyclodextrin-Functionalized Curcumin-Loaded Silver Nanoparticles

作者：陈建平[1,2];余苗[3];秦小明[1];谌素华[1];钟赛意[1]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]广东省天然产物绿色加工与产品安全重点实验室,广东广州510640;[3]江西省粮油质量监督检验中心,江西南昌330046

年份：2017

卷号：38

期号：21

起止页码：38

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD、、北大核心2014、CSCD_E2017_2018、EI(收录号：20174804460198)、Scopus、北大核心、CSCD

基金：国家自然科学基金青年科学基金项目(21602034);广东省自然科学基金博士启动项目(2016A30310332);广东省公益研究与能力建设项目(2015A020209166);广东省天然产物绿色加工与产品安全重点实验室开放基金项目(201617)

语种：中文

中文关键词：姜黄素;β-环糊精;银纳米粒子;制备;抗肿瘤活性

外文关键词：curcumin; β-cyclodextrin; silver nanoparticles; preparation; anticancer activity

中文摘要：采用纳米银材料制备负载姜黄素(curcumin,Cur)的β-环糊精(β-cyclodextrin,β-CD)功能化纳米银——Ag@β-CD@Cur,并进一步对其抗肿瘤活性进行测定。运用透射电子显微镜和纳米粒度仪鉴定Ag@β-CD@Cur的表面形貌、粒径大小和稳定性。运用倒置荧光显微镜和3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法检测Ag@β-CD@Cur对人肝癌细胞HepG2形态和活性的影响,并进一步采用流式细胞术检测细胞凋亡比例。实验结果表明,所制备的Ag@β-CD@Cur是纯度很高的单质银,且大小均一、表面光滑,粒径约为2 nm。同时,Ag@β-CD@Cur具有较好的稳定性,能在水中稳定存在30 d。经Ag@β-CD@Cur处理HepG2细胞24 h后,HepG2细胞的形态出现明显的变圆、细胞核固缩等细胞凋亡特征,同时,细胞存活率从对照组的100%降低到24%,进一步的流式细胞术分析结果表明,细胞凋亡比例从对照组的2.5%增加到51.0%。实验结果表明,纳米化后的Cur具有较强的抗肿瘤活性效果,这为Cur在纳米制剂的应用提供了一定的技术手段和理论数据参考。

外文摘要：In this study, cyclodextrin-functionalized curcumin-loaded silver nanoparticles （Ag@β-CD@Cur） were prepared with silver nanomaterials and its anticancer activity was also studied. The surface morphology, particle size and stability of Ag@β-CD@Cur were detected by transmission electronic microscopy and Zetasizer Nano. Moreover, the effect of Ag@β-CD@Cur on morphological and viability changes of HepG2 cells were detected by phase-contrast microscopy and （3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide assay. Moreover, the population of apoptotic cells was detected by ？ow cytometric analysis. The experimental results showed that Ag@β-CD@Cur was obtained as highly pure, uniformly sized silver nanoparticles with a smooth surface. The average size of Ag@β-CD@Cur was 2 nm. Moreover, Ag@β-CD@Cur had good stability in water for 30 days. After treatment of HepG2 cells with Ag@β-CD@Cur for 24 h, apoptosis characteristics appeared such as cell swelling and cell nuclei pyknosis. At the same time, the cell viability reduced to 24% of the control group. Further ？ow cytometric analysis showed that the population of apoptotic cells increased from 2.5% （control） to 51.0%. The results demonstrated that after nanoparticlization, curcumin has stronger anticancer activity, which will provide a technical means and theoretical data for curcumin nano-formulation.