文献类型：期刊文献

英文题名：LC–MS/MS-Based Metabolome Analysis of Biochemical Pathways Altered by Food Limitation in Larvae of Ivory Shell, Babylonia areolata

作者：Fu, Jingqiang[1,2]; Shen, Minghui[1,2,3]; Shen, Yawei[1,2]; Lü, Wengang[4]; Huang, Miaoqin[1,2]; Luo, Xuan[1,2,5]; Yu, Jinjin[1,2]; Ke, Caihuan[1,2,5]; You, Weiwei[1,2,5]

机构：[1] State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361102, China; [2] College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China; [3] Tropical Marine Products Fine Breed Center, Hainan Academy of Ocean and Fisheries Sciences, Hainan, 570216, China; [4] College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, China; [5] Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, China

年份：2018

卷号：20

期号：4

起止页码：451

外文期刊名：Marine Biotechnology

收录：EI(收录号：20181705049981)

语种：英文

外文关键词：Metabolites - Principal component analysis - Biomolecules - Disintegration - Marine biology - Polyunsaturated fatty acids - Phospholipids - Cluster analysis - Nutrients

外文摘要：Ivory shell, Babylonia areolata, is one of the commercially important mariculture species in China and South East Asia. Survival varies in the artificial hatching and larval rearing of B. areolata. Food deprivation may be involved in rearing mortality, and so, a better understanding of how larvae respond and adjust to starvation is needed. In this study, the metabolite profiles of newly hatched larvae with yolk (I), larvae with yolk exhaustion (II), larvae suffering 24?h starvation after yolk exhaustion (III), and larvae fed with exogenous nutrients after yolk exhaustion (IV) were analyzed by LC–MS/MS. Principal component and cluster analyses revealed differential abundance of metabolite profiles across groups. When compared to metabolite levels of the I group, significantly up-regulated metabolites included polyunsaturated fatty acids, phospholipids, nucleotide, amino acids, and their derivatives were found in the II group, indicating that organisms relied predominantly on glycerophospolipid metabolism and protein-based catabolism for energy production during this stage. During starvation after yolk exhaustion, the levels of all energy related metabolites were significantly reduced, but an increase in products of purine and pyrimidine metabolism indicated an insufficient energy supply and an increase in cellular disintegration. Larvae fed exogenous nutrients can have significantly improved metabolism compared to starved larvae. These findings suggest that metabolomics, using LC–MS/MS, can be used to assess the physiological status and food-affected metabolic changes affecting B. areolata larvae. ? 2018, Springer Science+Business Media, LLC, part of Springer Nature.