文献类型：期刊文献

中文题名：双亲改性壳聚糖衍生物的制备及性能

英文题名：Preparation and properties of amphiphilic modified chitosan derivatives

作者：司桢羽[1];夏琳琳[1];胡章[1];陈梓茵[1];李诗纹[1]

机构：[1]广东海洋大学化学与环境学院,广东湛江524088

年份：2025

卷号：53

期号：3

起止页码：1

中文期刊名：工程塑料应用

外文期刊名：Engineering Plastics Application

收录：北大核心2023、、EI(收录号：20252018446363)、北大核心

基金：国家重点研发计划项目(2020YFD0901101);广东省自然科学基金项目(2024A1515012618);广东省普通高校重点项目(2022ZDZX2027);大学生创新创业训练计划项目(S202310566059);广东海洋大学大学生创新团队项目(CXTD2021020)。

语种：中文

中文关键词：壳聚糖;双亲改性;表面电势;临界胶束浓度;抗菌活性

外文关键词：chitosan;amphiphilic modification;surface potential;critical micelle concentration;antibacterial activity

中文摘要：为了获得新型抗菌活性化合物,模仿抗菌肽的带正电性和两亲性结构,对海洋来源壳聚糖(CS)进行化学改性。通过CS与2-氯乙胺盐酸盐的亲核取代反应对CS进行亲水改性,得到亲水性CS(HCS),随后通过HCS与L-异亮氨酸的酰胺化反应进行疏水性修饰,获得系列双亲改性CS衍生物,即双亲性CS(ACS)。采用紫外-可见光谱、傅里叶变换红外光谱、X射线衍射以及Zeta电位仪对制备的样品进行结构表征;通过测定临界胶束浓度,考察ACS的自组装性能;采用抑菌圈法对ACS的体外抗菌效果进行评价。结果表明,双亲改性CS衍生物已成功制备,其表面电势都呈正电性,Zeta电位范围为35.6~39.2 mV,临界胶束浓度值为0.16~0.28 g/L,表明ACS具有较强的胶束形成能力;ACS具有强的抗菌活性,其中HCS与L-异亮氨酸质量比为1.5∶1的ACS抗菌活性最强,对革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌的抑菌圈直径分别达21.2 mm和23.3 mm。因此,制备的新型双亲改性CS衍生物有望进一步开发为在食品、医药、农业等诸多领域广泛应用的抗菌材料。

外文摘要：To obtain new antibacterial compounds,chemical modification of marine derived chitosan(CS)was carried out by imitating the positively charged amphiphilic structure of antimicrobial peptides.Hydrophilic modification of CS was carried out through nucleophilic substitution reaction of CS with 2-chloroethylamine hydrochloride for preparing hydrophilic CS(HCS),then a series of amphiphilic modified CS derivatives,namely amphiphilic CS(ACS),were prepared by hydrophobic modification through amidation reaction of HCS with L-isoleucine.The structure of the prepared samples was characterized by using ultraviolet-visible spectroscopy,Fourier transform infrared spectroscopy,X-ray diffraction spectroscopy and a Zeta potential instrument.The selfassembly properties of ACS were investigated by determining the critical micelle concentrations,and the antibacterial effects of ACS were evaluated in vitro using the inhibition zone method.The results show that ACS are successfully prepared,and the surface potentials of ACS are positively charged,with a zeta potential range of 35.6-39.2 mV.The critical micelle concentration values of ACS range from 0.16-0.28 g/L,indicating strong micelle formation ability.ACS have excellent antibacterial activity,among them,ACS with the mass ratio of HCS to L-isoleucine of 1.5∶1 has the strongest antibacterial activity,with inhibition zone diameters of 21.2 mm and 23.3 mm against Gram negative Escherichia coli and Gram positive Staphylococcus aureus,respectively.Therefore,the prepared novel amphiphilic modified CS derivatives are expected to be further developed into antibacterial materials widely used in many fields,such as food,medicine,and agriculture.