文献类型：期刊文献

英文题名：κ-carrageenan oligosaccharides alleviate MDP induced rumen epithelial cell inflammatory damage by inhibiting the activation of NOD2/NF-κB pathway

作者：Xiao, Yimei[1,2];He, Xiaolin[1,2];Ma, Jian[1];Du, Chunmei[1];Gan, Shangquan[1];Yin, Fuquan[1,2]

机构：[1]Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Dept Anim Sci, Key Lab Anim Resources & Breed Innovat Western Gua, Zhanjiang, Peoples R China

年份：2025

卷号：12

外文期刊名：FRONTIERS IN VETERINARY SCIENCE

收录：SCI-EXPANDED(收录号：WOS:001533995500001)、、WOS

基金：The authors would like to thank all people who were involved in the trial. The authors are grateful for the financial support from the Agriculture and Rural Affairs of Guangdong Department.

语种：英文

外文关键词：kappa-carrageenan oligosaccharides; inflammation; muramyl dipeptide; ruminal epithelial cells; NOD2/NF-kappa B signaling pathway

外文摘要：Introduction Under Subacute Ruminal Acidosis (SARA) conditions, harmful substances released by the massive lysis of ruminal bacteria are further degraded into small bacterial peptides, such as muramyl dipeptide (MDP). These degradation products are absorbed through the rumen wall and enter the bloodstream continuously, triggering a series of nutritional metabolic diseases and systemic pro-inflammatory responses. Therefore, inhibiting MDP-induced damage emerges as a novel target for preventing and alleviating SARA. Nutritional regulation serves as a critical strategy for enhancing the body's resistance to inflammatory challenges. Moreover, plant-derived oligosaccharide extracts offer a promising approach for the prevention and control of animal diseases.Methods In this study, the protective effects and possible molecular mechanisms of kappa-carrageenan oligosaccharides (KOS) against MDP-induced damage in ovine ruminal epithelial cells (ORECs) were evaluated. The CCK8 assay, western blot analysis, ELISA, and PCR were employed in this study.Results The results demonstrated that exposing ORECs to 25 mu g/mL MDP for 6 h induced inflammatory damage. In contrast, pretreatment of ORECs with 75 mu g/mL KOS for 9 h significantly enhanced cell viability, downregulated pro-inflammatory cytokine levels, restored immunoglobulin concentrations, reduced apoptosis rates, and regulated the expression of apoptosis-related genes under MDP stimulation. KOS exerted anti-inflammatory effects by scavenging ROS, improving tight junction barrier function, and inhibiting activation of the NOD2/NF-kappa B signaling pathway.Conclusion Pretreatment with 75 mu g/mL KOS for 9 h effectively alleviated MDP-induced inflammatory damage in ORECs by inhibiting the activation of the NOD2/NF-kappa B pathway.