文献类型：期刊文献

中文题名：超声联合复合保水剂对冷冻皱纹盘鲍腹足肌质地改良及其机制

英文题名：Texture Improvement of Frozen Gastropod Muscle of Abalone(Haliotis disus Hannai)by Ultrasound Combined with Composite Water-retaining Agent and Its Mechanism

作者：陈婉绮[1];张锡立[1];马伟雄[3];陈忠琴[1,2];曹文红[1,2];林海生[1,2];高加龙[1,2];谭明堂[1,2];郑惠娜[1,2]

机构：[1]广东省水产品加工与安全重点实验室,广东海洋大学食品科技学院,广东湛江524088;[2]广东海洋大学深圳研究院,广东深圳518108;[3]广东环球水产食品有限公司,广东茂名525000

年份：2025

卷号：45

期号：4

起止页码：127

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：北大核心2023、、北大核心

基金：广东省现代农业产业技术体系贝藻创新团队项目(2024CXTD23);现代农业产业技术体系项目(CARS-49)。

语种：中文

中文关键词：皱纹盘鲍;复合保水剂;超声;质地改良;蛋白质组学

外文关键词：Haliotiis disus Hannai;compound water retention agent;ultrasound;texture improvement;proteomics

中文摘要：【目的】研究联合嫩化技术对冷冻皱纹盘鲍(Haliotis disus Hannai)腹足肌热加工质地的改良及其机制。【方法】基于复合保水剂浸泡结合超声波联合嫩化处理冷冻皱纹盘鲍腹足肌,采用质构分析联合嫩化处理对鲍腹足肌的嫩化效果,进一步采用低场核磁、电子扫描电镜(SEM)、聚丙烯酰胺凝胶(SDS-PAGE)电泳、蛋白质组学等方法研究其嫩化机制。【结果与结论】复合保水剂联合超声处理明显降低鲍腹足肌硬度,使肌肉不易流动水含量提高,并使肌原纤维断裂且显著膨胀,断裂膨胀的肌纤维与淀粉糊化颗粒物形成更小的纤维间隙。肌原纤维蛋白中肌球蛋白重链(200 ku)、副肌球蛋白(100 ku)、肌钙蛋白(37 ku)降解,胶原蛋白仅高分子质量蛋白条带(>275 ku)发生降解。联合处理改变两种蛋白质的三级结构,表现为表面疏水性提高,巯基被氧化形成二硫键及蛋白质羰基化加剧。蛋白质组学结果显示,联合处理后,上调蛋白主要包括肌原纤维蛋白相关蛋白、胶原蛋白相关蛋白和蛋白质降解合成相关蛋白,下调蛋白包括热休克蛋白、结构蛋白相关蛋白、相关酶类及卵黄素等。超声联合复合保水剂通过超声的空化效应破坏肌纤维结构并加速保水剂渗透,同时保水剂通过氢键作用提高肌肉持水性,二者协同促进肌原纤维蛋白和胶原蛋白降解,改变蛋白质三级结构,实现鲍腹足肌质地改良。

外文摘要：【Objective】This study aims to the improvement of the thermal processing texture of frozen abalone(Haliotis disos Hannai)gastropod muscle by combined tenderization technology and its mechanism.【Method】Based on the combined tenderization treatment of frozen abalone gastropod muscle by soaking with a composite water-retaining agent combined with ultrasound,texture analysis was employed to investigate the tenderization effect for abalone gastropod muscle,and the tenderization mechanism was further studied by Low-field NMR,SEM,SDS-PAGE electrophoresis and proteomics.【Result and Conclusion】The combination of tenderizer and ultrasonic treatment significantly reduced the hardness of abalone gastropod muscles,and increased the content of water in the muscles that was not easy to flow,and caused the myofibrils broke and swelled significantly,and the broken and expanded muscle fibers formed a smaller fiber gap with starch gelatinized particles.In myofibrillar protein,myosin heavy chain(200 ku),para myosin(100 ku),and troponin(37 ku)were degraded,while in collagen,only high molecular weight protein band(>275 ku)was degraded.The combined treatment changed the tertiary structure of the two proteins,resulting in increased surface hydrophobicity,oxidation of thiol groups to form disulfide bonds,and intensified protein carbonylation.The proteomics results showed that after the combined treatment,the up-regulated proteins mainly included myofibrillar protein-related proteins,collagen-related proteins and protein degradation and synthesis-related proteins,and the down-regulated proteins included heat shock proteins,structural protein-related proteins,related enzymes and vitelline.Ultrasound combined with compound water retention agent destroys the structure of muscle fibers and accelerates the penetration of water retention agent through the cavitation effect of ultrasound,while the water retention agent improves muscle water holding capacity through hydrogen bonding,which synergistically promotes the degradation of myofibrillar protein and collagen,changes the tertiary structure of protein,and improves the texture of gastropod muscle.