文献类型：期刊文献

英文题名：A sensitive fluorometric sensor for Ag+based on the hybridization chain reaction coupled with a glucose oxidase dual-signal amplification strategy

作者：Li, Yubin[1]; Xie, Ling[1]; Yuan, Jiaming[1]; Liu, Huazhong[1]

机构：[1] College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, 524088, China

年份：2020

卷号：10

期号：44

起止页码：26239

外文期刊名：RSC Advances

收录：EI(收录号：20203409079235)、Scopus(收录号：2-s2.0-85089470633)

语种：英文

外文关键词：Metal ions - Silver - Glucose sensors - Coenzymes - Propionic acid - Signal processing - Glucose - Glucose oxidase

外文摘要：In this work, an efficient and sensitive fluorometric sensor was developed to detect silver ions (Ag+). It is based on the cytosine-Ag+-cytosine (C-Ag+-C) structure via a dual-signal amplification strategy using glucose oxidase (GOx) and the hybridization chain reaction (HCR). A silver-coated glass slide (SCGS) acts as an ideal material for separation. Cytosine rich (C-rich) capture DNA (C-DNA) assembled themselves on the SCGS via Ag-S bonds and hybridized with signal DNA (S-DNA) to trigger the HCR. With specific base-pairing, the S-DNA and HCR products bind on the SCGS. Then, the GOx-biotin-streptavidin (SA) complexes bind to the HCR products through SA-biotin interactions. Owing to the formation of a particular C-Ag+-C structure between two neighboring C-rich C-DNA on the SCGS, the C-DNA/S-DNA/HP1-GOx/HP2-GOx complex gradually moved away from the SCGS as the concentration of Ag+ increased and the combined GOx fell into the buffer. H2O2 could be generated during the oxidation of glucose, catalyzed by GOx in the buffer. Afterward, H2O2 could oxidize the substrate (3-(p-hydroxyphenyl)-propanoic acid) when Horseradish peroxidase was present, giving rise to blue fluorescence. The proposed strategy reached a limit of detection (LOD) of 1.8 pmol L-1 with a linear detection range of 5 to 1000 pmol L-1 for Ag+. Moreover, this assay has been commendably used for the detection of Ag+ in actual samples with fairly good results. ? 2020 The Royal Society of Chemistry.