文献类型：期刊文献

英文题名：Effects of Dietary Alpha-Lipoic Acid on Growth Performance, Serum Biochemical Indexes, Liver Antioxidant Capacity and Transcriptome of Juvenile Hybrid Grouper (Epinephelus fuscoguttatus?x Epinephelus polyphekadion?)

作者：Ou, Guanghai[1];Xie, Ruitao[2];Huang, Jiansheng[1,3];Huang, Jianpeng[1];Wen, Zhenwei[1];Li, Yu[1];Jiang, Xintao[1];Ma, Qian[1,3];Chen, Gang[1,3]

机构：[1]Guangdong Ocean Univ, Fishery Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Evergreen Feed Ind Co Ltd, Zhanjiang 524000, Peoples R China;[3]Guangdong Prov Key Lab Pathogen Biol & Epidemiol A, Zhanjiang 524088, Peoples R China

年份：2023

卷号：13

期号：5

外文期刊名：ANIMALS

收录：SCI-EXPANDED(收录号：WOS:000947450400001)、、WOS

基金：This work was financially supported by National Key R&D Program Blue Granary Science and Technology Innovation Key Special Project (2020YFD0900204) and the National Key R&D Program of China (2020YFD0900200).

语种：英文

外文关键词：juvenile hybrid grouper; alpha-lipoic acid; growth performance; serum biochemical indexes; antioxidant capacity; transcriptome

外文摘要：We aimed to investigate the effects of dietary alpha-lipoic acid (cc -LA) on the growth performance, serum biochemical indexes, liver morphology, antioxidant capacity, and transcriptome of juvenile hybrid groupers (Epinephelus fuscoguttatus?x Epinephelus polyphekadion?). Four experimental diets supplemented with 0 (SL0), 0.4 (L1), 0.6 (L2), and 1.2 (L3) g/kg cc -LA were formulated and fed to three replicates of juvenile hybrid grouper (24.06 +/- 0.15 g) for 56 d. The results indicated that dietary 0.4 and 0.6 g/kg cc -LA significantly decreased the weight gain rate in juvenile hybrid groupers. Compared with SL0, the content of total protein in the serum of L1, L2, and L3 increased significantly, and alanine aminotransferase decreased significantly. The content of albumin in the serum of L3 increased significantly, and triglyceride, total cholesterol, and aspartate aminotransferase decreased significantly. In addition, the hepatocyte morphology in L1, L2, and L3 all showed varying degrees of improvement, and the activities of glutathione peroxidase and superoxide dismutase in the liver of L2 and L3 were significantly increased. A total of 42 differentially expressed genes were screened in the transcriptome data. KEGG showed that a total of 12 pathways were significantly enriched, including the pathway related to immune function and glucose homeostasis. The expression of genes (ifnk, prl4a1, prl3b1, and ctsl) related to immune were significantly up-regulated, and the expressions of gapdh and eno1 genes related to glucose homeostasis were significantly down-regulated and up regulated, respectively. In summary, dietary supplementation of 0.4 and 0.6 g/kg cc -LA inhibited the growth performance of juvenile hybrid groupers. A total of 1.2 g/kg cc -LA could reduce the blood lipid level, improve hepatocyte damage, and increase the hepatic antioxidant enzyme activity. Dietary cc -LA significantly affected the pathway related to immune function and glucose homeostasis.