文献类型：期刊文献

中文题名：猪笼草的组织培养

英文题名：TISSUE CULTURE AND RAPID PROPAGATION OF NEPENTHES MIRABILIS

作者：丰锋[1];李洪波[1];谢建英[1]

机构：[1]湛江海洋大学农学院,广东湛江524088

年份：2002

卷号：24

期号：3

起止页码：268

中文期刊名：西南农业大学学报（自然科学版）

外文期刊名：Journal of Southwest Agricultural University

收录：CSTPCD、、北大核心2000、CSCD_E2011_2012、北大核心、CSCD

基金：湛江市科委资助项目

语种：中文

中文关键词：猪笼草;组织培养;繁殖;腋芽;春季;培养基;液体培养

外文关键词：Nepenthes mirabilis ; tissue culture; propagation

中文摘要：以猪笼草 (Nepenthesmirabilis)腋芽为试材 ,研究猪笼草的组培快繁 ,结果表明 ,外殖体的建立以 1/ 2MS +6 -BA1+NAA0 .0 5培养基液体培养春季 (3～ 6月 )进行最佳。愈伤组织 (或不定芽 )增殖以MS +6 -BA1+Ad10 +NAA0 .1+10 0ml/L椰子汁最佳 ,高浓度 (2 0 0ml/L)的椰子汁不利于愈伤组织 (或不定芽 )增殖 ;继代芽的增殖以MS +6 -BA1.5+Ad1～ 5+NAA0 .1+10 0～ 2 0 0ml/L椰子汁最好 ;生根培养用 1/ 2MS +6 -BA0 .1+NAA1.0 ,2 0d后可长出 2～

外文摘要：Axillary buds of Nepenthes mirabilis were used in tissue culture in the spring and autumn of 1997～1999 to investigate the optimum conditions for the rapid propagation of the species. Culture on 1/2 MS liquid medium supplemented with 6-BA 1 mg/L and NAA 0.05 mg/L in March to May gave the best results for the production of virus-free materials. The optimum medium for proliferation of calli (or adventitious buds) appeared to be MS + 6-BA 1 mg/L +Ad 10 mg/L + NAA 0.1 mg/L + coconut milk 100 ml/L, and higher concentration of coconut milk (200 ml/L) showed an inhibitive effect. Best proliferation of the subcultured buds was obtained on the medium MS + 6-BA 1.5 mg/L + Ad 1～5 mg/L + NAA 0.2 mg/L + coconut milk 100～200 ml/L. Cultured on 1/2 MS + 6-BA 0.1 mg/L + NAA 1.0 mg/L, the regenerated shoots rooted readily in 20 days.