文献类型：期刊文献

中文题名：β-葡聚糖对奥尼罗非鱼生长性能及抗嗜水气单胞菌感染的影响

英文题名：Effects of dietary β-glucan on growth performance and disease resistance of juvenile hybrid tilapia(Oreochromis aureus ♂×Oreochromis niloticus♀)

作者：迟淑艳[1];周歧存[1];周健斌[1];杨奇慧[1];董晓慧[1]

机构：[1]广东海洋大学水产学院

年份：2006

卷号：13

期号：5

起止页码：767

中文期刊名：中国水产科学

外文期刊名：Journal of Fishery Sciences of China

收录：CSTPCD、、北大核心2004、Scopus、CSCD2011_2012、北大核心、CSCD

基金：广东省科技攻关项目(2004B10401006);广东省重大科技兴渔项目(B200309D01)

语种：中文

中文关键词：奥尼罗非鱼;β-葡聚糖;生长性能;抗病力

外文关键词：Oreochromis aureus♂×Oreochromis niloticus♀;β-glucan performance;disease resistance

中文摘要：本实验采用初始体质量（1.84±0.02）g的全雄奥尼罗非鱼（Oreochromis aureus♂×Oreochromis niloticus♀）作为饲养对象，在饲料中分别添加不同水平的β-葡聚糖（添加量分别为0、0.5％、1.0％、1.5％、2％和4％），共设6个处理，饲养8周，研究β-葡聚糖对奥尼罗非鱼增重率、特定生长率和成活率等生长指标的影响。8周后注射嗜水气单胞菌（Aerornonas hydrophila）进行感染实验，研究β-葡聚糖对奥尼罗非鱼存活率和免疫调节的影响。观察7天，计算存活率，测定头肾的酸性磷酸酶（ACP）和溶菌酶（L5Z）活性。结果显示，饲养8周后各组成活率为96.67％～100％，添加β-葡聚糖对增重率、特定生长率、饵料转化率和蛋白质效率影响不显著，1.5％添加组的肥满度显著高于其他各组（P〈0.05），2.0％添加组奥尼罗非鱼肌肉蛋白含量显著高出对照组和0.5％组分别为3.76和3.02个百分点（P〈0.05）；注射嗜水气单胞菌7天后，对照组的存活率仅为25％，β-葡聚糖添加组的存活率在60％～70％，1.5％的β-葡聚糖添加组可以显著提高（P〈0.05）奥尼罗非鱼头肾组织ACP和LSZ的活性。结论，全雄奥尼罗非鱼饲料中β-葡聚糖的适宜添加量在1.0％～1.5％时，可以明显改善其生长性能和抗嗜水气单胞菌感染的能力。

外文摘要：One thousand hybridization tilapia（Oreochromis aureus♂×Oreochromis niloticus♀） were fed with the basal diet for 7 days. On day 8, five hundreds forty fish[ initial weight（ 1.84±0.02）g] were selected and randomly divided into 6 treatments with 3 replicates of 30 fish each for the feeding test. β-（ 1,3）-glucan was administered to the hybridization tilapia through the diets at six levels（0,0.5 96,1.0%, 1.5 %, 2.0 % ,4.0 %, respectively） as measured the weight gain rate（WGR）, special growth rate（SGR）, survival rate（SR） and nutrients of muscle. The feed test period was 8 weeks. At the end of the feeding test,the fish were selected, 10 in each replicate randomly for infection test for 7 days. Effects of β-（ 1,3）-glucan administration on survival and immune modulations were studied in the fish against the bacterial pathogen, Aeromonas hydrophila by injection. SR was determined at different times after injection（1h、24h、48h、96h. 168h, respectively）. On day 7, test animals injected bacterial pathogen were collected to measure the acid phosphates（ACP） activity and lysozyme（LSZ） activity of head kidney. The results showed that after 56 days of feeding, survival rate ranged from 96.67%～100% and WGR, SGR and SR were not significantly different（P〉0.05 ） among dietary treatments. The condition factor of the animals fed on 1.5%β-glucan was significantly higher than the others（P〈0.05）. The muscle protein contents of the fish fed on 2.0% β-glucan were 3.76% and 3.02% higher than the control and 0.5% group, respectively（P〈0.05 ）. On day 7 after injection of Aeromonas hydrophila, SR of the control was only 25%, and that of β-glucan supplementation groups were between 60% and 70%. The acid phosphates activity and lysozyme activity of head kidney were significantly higher in the hybridization tilapias fed on 1.5% β-glucan. Conclusion, in this test, 1.0 β-1.5% β-glucan supplementation in the dietary of hybridization tilapia was recommended considering the performance and disease resistance.