文献类型：期刊文献

英文题名：Identification and functional characterization of a c-type lysozyme from Fenneropenaeus penicillatus

作者：Cai, Shuanghu[1,2];Zhang, Yilin[1,2];Wu, Fan[1,2];Wu, Rimin[1,2];Yang, Shiping[1,2];Li, Ya[3];Xu, Youhou[4]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Guangdong Higher Educ Inst, Fisheries Coll, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Key Lab Control Dis Aquat Econ Anim, Guangdong Higher Educ Inst, Fisheries Coll, Zhanjiang, Peoples R China;[3]Guangdong Ocean Univ, Agr Coll, Zhanjiang, Peoples R China;[4]Beibu Gulf Univ, Guangxi Key Lab Beibu Gulf Marine Biodivers Conse, 89 West St Nanzhu, Qinzhou, Guangxi, Peoples R China

年份：2019

卷号：88

起止页码：161

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000465060500019)、、WOS

基金：This study was supported by the National Science Foundation of China (Grant No. 31572656), National Science Foundation of Guangdong Province (No. 2017A030307033), Innovative Training Program of Guangdong Ocean University (CXXL2018047) and Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University.

语种：英文

外文关键词：Fenneropenaeus penicillatus; c-type lysozyme; WSSV; Vibrio alginolyticus; Expression pattern

外文摘要：Lysozyme is an important defense molecule of the innate immune system and possess high antimicrobial activities. In this study, a full-length c-type lysozyme cDNA (Fplysc) was cloned and characterized from Fenneropenaeus penicillants. The cDNA contains an open reading frame of 477 bp encoding 158 amino acids, with 53-94% identity with those of other crustaceans. The recombinant Fplysc had antibacterial activities against Gram-positive bacteria (Streptococcus agalactiae and Micrococcus luteus) and Gram-negative bacteria (Vibrio alginolyticus and Escherichia colt), and showed antiviral activity against WSSV and IHHNV. The qRT-PCR analysis showed that Fplysc expression levels were most abundant in hemocytes and less in eyestalk. The expression levels of Fplysc were significantly upregulated in gill, intestine and hemocytes when challenged with WSSV and V. alginolyticus. Fplysc-silencling suppressed Fplysc expression in cephalothoraxes and increased mortality caused by WSSV and V. alginolyticus, and exogenous rFplysc led to a significant decrease of shrimp mortality by injecting rFplysc into Pplysc silenced shrimp, suggesting Fplysc is the important molecule in shrimp antimicrobial and antiviral response. In conclusion, the results provide some insights into the function of Fplysc in shrimp against bacterial and viral infection.