文献类型：期刊文献

英文题名：Partial substitution of fish meal by Clostridium autoethanogenum protein in the diets of juvenile largemouth bass (Micropterus salmoides)

作者：Zhu, Shujie[1];Gao, Weihua[1];Wen, Zhengyong[3];Chi, Shuyan[2];Shi, Yuhui[1];Hu, Wei[1];Tan, Beiping[2]

机构：[1]Yangtze Univ, Hubei Key Lab Waterlogging Disaster & Agr Use Wet, Jingzhou, Peoples R China;[2]Guangdong Ocean Univ, Guangdong South China Sea Key Lab Aquaculture Aqu, Zhanjiang, Peoples R China;[3]Neijiang Normal Univ, Key Lab Sichuan Prov Fishes Conservat & Utilizat, Neijiang, Sichuan, Peoples R China

年份：2022

卷号：22

外文期刊名：AQUACULTURE REPORTS

收录：SCI-EXPANDED(收录号：WOS:000720613100001)、、Scopus(收录号：2-s2.0-85118863237)、WOS

基金：This work was supported by National Key R&D Program of China, China [grant numbers 2019YFD0900200] ; Research and Demonstration of Precision Functional Compound Feed Technology of Major Cultured Fishes and Shrimps in South China, China [2021B0202050002] ; the Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals, Guangdong Ocean University, China [grant numbers KFKT2019YB03] ; and Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, China [grant numbers NJTCCJSYSYS02] .

语种：英文

外文关键词：Clostridium autoethanogenum protein; Growth performance; Digestive capacity; Antioxidant index; Micropterus salmoides

外文摘要：The present study was conducted to investigate the effect of fish meal replacement with Clostridium autoethanogenum protein (CAP) on growth performance, haemato-biochemistry, digestive capacity, antioxidant index, and histopathological examination of liver and intestine in largemouth bass (Micropterus salmoides). Seven experimental diets were formulated with CAP replacement of 0% (CAP0), 15% (CAP15), 27% (CAP27), 39% (CAP39), 51% (CAP51), 63% (CAP63) and 75% (CAP75) fish meal, respectively. Results showed that replacement of fish meal by CAP did not affect SR, FBW, WGR, SGR and CF. While the fish fed diets containing CAP displayed a higher PER and a lower FCR than the control group (P < 0.05). However, the CAP inclusion level did not statistically affect the whole-body composition (P > 0.05). Additionally, The ADC of dry matter and protein were improved by addition of CAP (P < 0.05), while corresponding ADC of lipid were not significantly changed among different groups (P > 0.05). The lipase and amylase activities were not dramatically altered in stomach (P > 0.05), but were significantly increased in intestine with dietary substitution level up to 39% (P < 0.05). Differently, the protease activity of fish fed CAP75 was higher than other groups in stomach and intestine (P < 0.05). In addition, the activities of T-AOC and SOD in serum, liver and intestine were elevated with dietary CAP level (P < 0.05), whereas MDA content displayed the opposite trend. Moreover, the increased TP, ALB, TC, TG and AST/ALT were observed in treatment groups. In addition, the villus length and width of hindgut were dramatically increased with increasing dietary substitution level up to 39%. Furthermore, no adverse effect on the hepatic histology was occurred when fed diet with substitution level below 63%. Overall, the optimal CAP replacement level was 49.80% with maximum weight gain rate of juvenile largemouth bass.