文献类型：期刊文献

中文题名：脂肪酶水解金枪鱼油的工艺优化及脂质成分分析

英文题名：Process Optimization and Lipid Composition Analysis of Tuna Oil Hydrolyzed by Lipase

作者：李艳阳[1];王春艳[1];罗婷予[1];夏秋瑜[1,2];刘书成[1,2];单凯[3]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研发中心,广东省海洋生物制品工程重点实验室,水产品深加工广东普通高校重点实验室,广东湛江524088;[2]南方海洋科学与工程广东省实验室(湛江),广东湛江524025;[3]蔚蓝生物集团有限公司(无锡),江苏无锡214000

年份：2024

卷号：44

期号：6

起止页码：109

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：北大核心2023、、CSCD_E2023_2024、北大核心、CSCD

基金：国家自然科学基金(32172252)。

语种：中文

中文关键词：金枪鱼油;酶法水解;Omega-3多不饱和脂肪酸;工艺优化;脂质成分

外文关键词：tuna oil;enzymatic hydrolysis;omega-3 polyunsaturated fatty acids;process optimization;lipid composition

中文摘要：【目的】对固定化脂肪酶Rhizopus oryzae lipase(ROL)水解金枪鱼油进行工艺优化,并分析水解前后脂质组分差异。【方法】使用固定化脂肪酶ROL、RIM、ZT水解金枪鱼油,测定甘油酯的脂肪酸组成及脂质组成,筛选脂肪酶;用单因素实验研究温度、pH、水解转速对金枪鱼油水解度的影响,以及吸附剂、离心转速和离心时间等因素对水解甘油酯分离得率的影响;分别以水解度和得率为指标对单因素确定的最佳水平范围进行三因素三水平正交实验,并分析鱼油水解8h所得水解甘油酯的脂肪酸组成、脂质组成、热稳定性和氧化稳定性。【结果】经固定化脂肪酶ROL水解得到的甘油酯二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)富集效果最好,EPA和DHA质量分数达(42.11±1.69)%,Omega-3含量显著提高,甘油酯中甘油一酯和甘油二酯占比显著提升(P<0.05);金枪鱼油水解甘油酯的最佳水解条件为pH值7.5,温度40℃、转速300r/min,水解8h金枪鱼油水解度为(37.76±1.00)%;水解甘油酯提取过程中以油脂硅胶为吸附剂,最佳提取工艺为油脂硅胶与鱼油的质量比1∶150,离心时间5 min、离心转速6000 r/min,此条件下金枪鱼油甘油酯得率为(46.89±0.60)%,但热稳定性和氧化稳定性均降低,其中氧化稳定性变化显著(P<0.05)。【结论】固定化脂肪酶ROL水解金枪鱼油能显著改善甘油酯的脂质组成,提高EPA和DHA含量,以油脂硅胶为吸附剂的分离工艺可减少有机溶剂的使用,提高了经济性和环保性,优化的鱼油脂质水解和分离工艺为鱼油的精深加工和绿色处理提供了参考依据。

外文摘要：【Objective】To process optimization of the hydrolysis of tuna oil by immobilized lipase Rhizopus oryzae lipase(ROL)and to analyse the differences in lipid fractions before and after modification.【Methods】Immobilized lipases ROL,RIM and ZT were used to hydrolyze tuna oil and detecte the fatty acid composition and lipid composition to screen lipases;a one-way experiment was used to study the effects of temperature,or pH or hydrolysis speed on the hydrolysis degree of tuna oil;a one-way experiment was used to study the effects of adsorbents:fish oil(mass ratio),or centrifugal speed or centrifugal time on the yield of tuna oil;and the optimal level ranges ranges determined by the one-way experiments were tested by a three-factor,three-level,orthogonal experiment,using the degree of hydrolysis and the yield as the indexes,respectively.Orthogonal experiments were conducted to investigate the fatty acid composition,lipid composition,thermal stability and oxidative stability of hydrolysed glycerol esters with the same hydrolysis time(8 h).【Results】The immobilised lipase ROL with the best enrichment effect of EPA and DHA was selected,which mass fraction was(42.11±1.69)%.The monoglyceride and diglyceride in the glycerol ester hydrolysed by immobilised lipase ROL were significantly increased(P<0.05).The suitable hydrolysis conditions for hydrolysed glycerides of tuna oil were pH 7.5,temperature 40℃,rotational speed 300 r/min,and hydrolysis for 8 h.The hydrolysis degree of tuna oil under these conditions was(37.76±1.00)%.When hydrolysis of glycerol ester extraction processed with grease silica gel as the adsorbent,grease silica gel and fish oil mass ratio of 1∶150,centrifugation time of 5 min,centrifugal speed of 6000 r/min,and the hydrolysis of tuna oil after 8 h of hydrolysis,the yield of tuna oil glycerides after 8 h of hydrolysis was(46.89±0.60)%.Hydrolysis of tuna oil by immobilised lipase ROL significantly improved the lipid composition of the glycerides,but thermal oxidation stability is reduced and the oxidative stability of the glycerides of hydrolysed tuna oil was significantly reduced(P<0.05).【Conclusion】The optimised extraction process improved the lipid composition of glycerides and increased the content of EPA and DHA,reduced the use of organic reagents,Economical and environment-friendly,it provided a reference for the deep and green processing of fish oil.