文献类型：期刊文献

英文题名：Lycopene alleviates AFB₁-induced immunosuppression by inhibiting oxidative stress and apoptosis in the spleen of mice

作者：Xu, Feibo[1,2];Wang, Peiyan[1,2];Yao, Qiucheng[3];Shao, Bing[1];Yu, Hongyan[1,2];Yu, Kaiyuan[1,2];Li, Yanfei[1,2]

机构：[1]Northeast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R China;[2]Northeast Agr Univ, Dept Heilongjiang Common Anim Dis Prevent & Treat, Key Lab Prov Educ, Harbin 150030, Heilongjiang, Peoples R China;[3]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524000, Peoples R China

年份：2019

卷号：10

期号：7

起止页码：3868

外文期刊名：FOOD & FUNCTION

收录：SCI-EXPANDED(收录号：WOS:000476683600003)、、WOS

基金：This study was supported by a grant from the National Natural Science Foundation Project (31872530 and 31372496).

语种：英文

外文摘要：Lycopene (LYC) has been reported to exhibit antioxidant and immunoprotective activities, and our previous studies confirmed that LYC can alleviate multiple tissue damage induced by aflatoxin B1 (AFB(1)). However, it is unclear whether LYC could relieve the AFB(1)-induced immunosuppression. Thus, forty-eight male mice were randomly allocated and treated with LYC (5 mg kg(-1)) and/or AFB(1) (0.75 mg kg(-1)) by intragastric administration for 30 days. We found that LYC alleviated AFB(1)-induced immunosuppression by relieving splenic structure injury and increasing the spleen weight, spleen coefficient, T lymphocyte subsets, the contents of IL-2, IFN-gamma and TNF-alpha in serum, as well as the mRNA expression of IL-2, IFN-gamma and TNF-alpha in spleen. Furthermore, LYC inhibited oxidative stress induced by AFB(1)via decreasing the levels of reactive oxygen species (ROS), hydrogen peroxide (H2O2) and malondialdehyde (MDA), while enhancing the total antioxidant capacity (T-AOC) and antioxidant enzyme activities. In addition, LYC also restrained splenic apoptosis through blocking mitochondria-mediated apoptosis in AFB(1) intoxicated mice, presenting as the increase of mitochondrial membrane potential, and the decrease of cytoplasmic Cyt-c protein expression, cleaved Caspase-3 protein expression, Caspase-3/9 activities and mRNA expressions, as well as balancing the mitochondrial protein and mRNA expressions of Bax and Bcl-2. These results indicate that LYC can alleviate AFB(1)-induced immunosuppression by inhibiting oxidative stress and mitochondria-mediated apoptosis of mice spleen.