文献类型：期刊文献

中文题名：PCB126暴露对斑马鱼胚胎发育及氧化应激的影响

英文题名：Developmental toxicity and oxidative stress of PCB126 to zebrafish embryos

作者：刘寒[1];林红英[1];聂芳红[2];孔庆波[3];马驿[1];巨向红[1];宋春雨[1];陈进军[1];蔡吉榛[1]

机构：[1]广东海洋大学动物医学系,广东湛江524088;[2]广东海洋大学食品科技学院;[3]重庆警官职业学院侦查系

年份：2012

卷号：26

期号：1

起止页码：9

中文期刊名：毒理学杂志

外文期刊名：Journal of Toxicology

收录：CSTPCD、、北大核心2011、CSCD_E2011_2012、北大核心、CSCD

基金：广东省科技计划国际合作项目(2007B050200023;2010B050600004)

语种：中文

中文关键词：类二噁英多氯联苯;发育毒性;氧化应激;胚胎;斑马鱼

外文关键词：Ddioxin-like polychlorinated biphenyls; Developmental toxicity; Oxidative stress; Embryo; Zebrafish（Danio rerio）

中文摘要：目的探讨类二噁英多氯联苯PCB126通过氧化应激损伤介导的胚胎发育毒性机制。方法用不同浓度的PCB126(0、16、32、64和128μg/L)对斑马鱼胚胎进行水触媒染毒,检测PCB126对不同发育阶段斑马鱼胚胎存活率、心包卵黄囊水肿率、心率及铜锌超氧化物歧化酶(CuZn-SOD)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量的影响。结果从144 hpf后,随着PCB126浓度的增加,斑马鱼胚胎存活率明显下降(P<0.05),168 hpf时,128μg/L浓度组斑马鱼胚胎全部死亡;从96起,斑马鱼幼鱼心包卵黄囊水肿率显著增加(P<0.01),到144 hpf时,32μg/L浓度组水肿率达100%;在96和132 hpf时,128μg/L浓度组斑马鱼胚胎心率显著降低(P<0.01)。24 hpf时,各染毒组斑马鱼胚胎CuZn-SOD、CAT活性无明显变化,但64μg/L组MDA含量增加;72 hpf时,各染毒组CuZn-SOD活性降低,其中16和128μg/L组差异有统计学意义(P<0.01),64和128μg/L组CAT酶活性降低(P<0.05),32和128μg/L组MDA含量增加(P<0.05);96 hpf时,各染毒组斑马鱼CuZn-SOD及CAT活性均显著降低(P<0.01),32和128μg/L组MDA含量增加;132 hpf时,各染毒组斑马鱼胚胎CuZn-SOD和CAT活性均降低,MDA含量显著增加(P<0.01)。结论一定剂量的PCB126对斑马鱼胚胎发育具有致畸、致死作用,而氧化应激是其引起胚胎致畸、致死的重要机制之一。

外文摘要：Objective To probe into the embryotoxic mechanism mediated by oxidative stress of dioxin-like polychlorinated biphenyls（DlPCBs）.Methods A range of zebrafish（Danio rerio） embryos were waterbornely exposed to PCB126 at different concentrations of 0,16,32,64 and 128 μg/L,respectively.The survival rate,pericardium and yolk sac edema rate,heart rate as well as activities of copper-zinc superoxide dismutase（CuZn-SOD）,catalase（CAT） and content of malondialdehyde（MDA） of the embryos in different hours of poster-fertilization（hpf） were measured.Results The embryonic survival rate significantly decreased（P〈0.05） since 144 hpf as the concentrations of PCB126 increased,and all of the embryos exposed to 128 μg/L of PCB126 died at 168 hpf;The pericardium and yolk sac edema rate significantly increased（P〈0.01） since 96 hpf and the edema rate at 32 μg/L of PCB126 was 100% at 144 hpf;The heart rate at 128 μg/L of PCB126 significantly decreased at 96 hpf and 132 hpf（P〈0.01）,respectively.At 24 hpf,the activities of CuZn-SOD and CAT of the embryos in all of the exposure groups had no change while MDA of the embryos exposed to 64 μg/L of PCB126 increased;At 72 hpf,the activity of CuZn-SOD of the embryos in all of the exposure groups was enhanced,especially by far higher at 16 μg/L and 128 μg/L of PCB126 exposure（P〈0.01）,while the activity of CAT at 64 μg/L and 128 μg/L of PCB126 exposure was lowered（P〈0.05）,and MDA at 32 μg/L and 128 μg/L of PCB126 exposure increased（P〈0.05）,respectively.At 96 hpf,the activities of CuZn-SOD and CAT of the embryos in all of the exposure groups significantly decreased（P〈0.01） while MDA of the embryos exposed to 32 μg/L and 128 μg/L of PCB126 increased,respectively;At 132 hpf,the activities of CuZn-SOD and CAT of the embryos in all of the exposure groups significantly decreased（P〈0.01） and MDA of the embryos in all of the exposure groups significantly decreased（P〈0.01）,respectively.Conclusion PCB126 performed embryotoxicity including teratogenic and lethal effects to zebrafish embryos and the oxidative stress was one of the key mechanisms concerning the embryotoxicity.