文献类型：期刊文献

中文题名：人IgG诱导尼罗罗非鱼特异性抗体分泌细胞的ELISPOT检测

英文题名：Detection of Nile tilapia specific antibody secreting cells induced by human IgG via ELISPOT assay

作者：黄瑜[1,2,3];庞碧剑[1,2,3,4];李琦[1,2,3];吴灶和[2,3];简纪常[1,2,3]

机构：[1]广东海洋大学水产学院,广东湛江524088;[2]广东省水产动物病害防控与健康养殖重点实验室,广东湛江524088;[3]广东省教育厅水产经济动物病害控制重点实验室,广东湛江524088;[4]广西壮族自治区海洋环境监测中心站,广西北海536000

年份：2022

卷号：29

期号：10

起止页码：1417

中文期刊名：中国水产科学

外文期刊名：Journal of Fishery Sciences of China

收录：CSTPCD、、CSCD2021_2022、Scopus、北大核心、CSCD、北大核心2020

基金：国家自然科学基金项目(32002426);国家重点研发计划项目(2018YFD0900501)

语种：中文

中文关键词：尼罗罗非鱼;抗体分泌细胞;酶联免疫斑点法;抗体;免疫记忆

外文关键词：Nile tilapia;antibody secreting cell;ELISPOT;antibody;immune memory

中文摘要：通过酶联免疫斑点法(ELISPOT)检测了经人IgG免疫后尼罗罗非鱼(Oreochromis niloticus)头肾、外周血和脾脏中的特异性抗体分泌细胞(antibody secreting cell,ASC)数量。结果表明,首次免疫1 d后可在头肾中检测出ASC,而外周血和脾脏第3天才能检测出ASC;头肾、外周血以及脾脏中的ASC均在第12天达到峰值,随后头肾,外周血中的ASC数量显著减少,而在脾脏中ASC数量减少不显著。二次免疫1 d后在头肾、外周血和脾脏中均可检测到ASC,ASC数量均在第9天达到峰值,时间早于首次免疫,且在首次免疫和二次免疫中,头肾组织的ASC数量均是3个组织中最高的。酶联免疫吸附试验(ELISA)分别对首次免疫和二次免疫后血清中的抗体水平检测发现,其变化趋势与ASC数量变化规律相同。研究结果表明,尼罗罗非鱼在初次免疫后产生了免疫记忆,在二次免疫过程中产生了更多的ASC和抗体,头肾是ASC的主要来源组织。该结果可为鱼类的免疫记忆研究提供重要的科学依据。

外文摘要：Specific antibody secreting cells(ASC)play an important role in the immune response.In this study,to detect changes in specific ASCs in the Nile tilapia(Oreochromis niloticus)in response to exogenous stimuli,the number of ASCs in the head kidney,peripheral blood,and spleen of this fish after immunization with human IgG was detected by enzyme-linked immunospot assay(ELISPOT).The results showed that ASCs could be detected in the head kidney on day 1 after the first immunization,while ASC could be detected in the peripheral blood and spleen on day 3.The number of ASCs in the head kidney,peripheral blood,and spleen all reached their peak on day 12.While the number of ASCs in the kidney and peripheral blood was significantly reduced,that in the spleen was not significantly reduced.Subsequently,ASCs could be detected in the head kidney,peripheral blood,and spleen on day 1 after the second immunization,with the number of ASCs reaching their peak on day 9,earlier than the first immunization.In both the first and second immunizations,the number of ASCs in the head kidney tissue was the highest among the three tissues.Using enzyme linked immunosorbent assay(ELISA)to detect the antibody levels in serum after the first and second immunizations,the change trend was found to be the same as that of the ASCs.These results indicate that the Nile tilapia developed immune memory after the first immunization and produced more ASCs and antibodies during the second immunization.Furthermore,the head kidney was identified as the main source of ASCs.These findings provide an important scientific basis for the study of immune memory in fish.